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antibody phage display libraries contributions to oncology为肿瘤抗体噬菌体展示库贡献
Int. J. Mol. Sci. 2012, 13, 5420-5440; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Review
Antibody Phage Display Libraries: Contributions to Oncology
Carmela Dantas-Barbosa 1,*, Marcelo de Macedo Brigido 2 and Andrea Queiroz Maranhao 2
1 Centre de Recherche en Cancérologie de Lyon, UMR INSERM 1052-CNRS 5286, Centre Léon
Bérard, Cheney D, 28 Rue Laënnec, 69373 Lyon Cedex 08, France
2 Laboratório de Biologia Molecular, CEL/IB, Universidade de Brasília, Campus Darcy Ribeiro,
70910-900, Brasília-DF, Brazil; E-Mails: brigido@unb.br (M.d.M.B.);
andreaqm@unb.br (A.Q.M.)
* Author to whom correspondence should be addressed; E-Mail: carmeladantas@;
Tel.: +33-478-782-964; Fax: +33-478-782-720.
Received: 1 March 2012; in revised form: 5 April 2012 / Accepted: 24 April 2012 /
Published: 4 May 2012
Abstract: Since the advent of phage display technology, dating back to 1985, antibody
libraries displayed on filamentous phage surfaces have been used to identify specific
binders for many different purposes, including the recognition of tumors. Phage display
represents a high-throughput technique for screening billions of random fusion antibodies
against virtually any target on the surface or inside cancer cells, or even soluble markers
found in patient serum. Many phage display derived binders targeting import
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