antibody recognition of cancer-related gangliosides and their mimics investigated using in silico site mapping癌症相关的神经节甘脂抗体识别及其模拟研究使用计算机网站映射.pdfVIP

Antibody Recognition of Cancer-Related Gangliosides and Their Mimics Investigated Using in silico Site Mapping 1 1 2,3,4 Mark Agostino , Elizabeth Yuriev *, Paul A. Ramsland * 1 Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia, 2 Centre for Immunology, Burnet Institute, Melbourne, Victoria, Australia, 3 Department of Surgery Austin Health, University of Melbourne, Heidelberg, Victoria, Australia, 4 Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria, Australia Abstract Modified gangliosides may be overexpressed in certain types of cancer, thus, they are considered a valuable target in cancer immunotherapy. Structural knowledge of their interaction with antibodies is currently limited, due to the large size and high flexibility of these ligands. In this study, we apply our previously developed site mapping technique to investigate the recognition of cancer-related gangliosides by anti-ganglioside antibodies. The results reveal a potential ganglioside-binding motif in the four antibodies studied, suggesting the possibility of structural convergence in the anti-ganglioside immune response. The structural basis of the recognition of ganglioside-mimetic peptides is also investigated using site mapping and compared to ganglioside recognition. The peptides are shown to act as structural mimics of gangliosides by interacting with many of the same binding site residues as the cognate carbohydrate epitopes. These studies provide important clues as to the structural basis of immunological mimicry of carbohydrates. Citation: Agostino M, Yuriev E, Ramsland PA (2012) Antibody Recognition of Cancer-Related Gangliosides an