Celecoxib对糖尿病大鼠缺血再灌注脑组织NF-κB和ICAM-1表达的影响.docVIP

Celecoxib对糖尿病大鼠缺血再灌注脑组织NF-κB和ICAM-1表达的影响 祁学章1 范瑞明2 杨丽 2 余昌胤2 【摘要】目的 探讨celecoxib对糖尿病大鼠脑缺血再灌注后脑组织中NF-κB和ICAM-1表达的影响。方法 采用SD大鼠腹腔注射链脲佐菌素(STZ)和线栓法制作糖尿病大鼠大脑中动脉缺血再灌注模型。大鼠分为假手术组（S组）、脑缺血再灌注生理盐水组(NS组)、celecoxib低剂量组(LCIR组)和高剂量组（HCIR组），分别于缺血后30min给予生理盐水或celecoxib溶液灌胃，再灌注后6、12、24、48h将大鼠断头取脑，免疫组化法检测脑组织中NF-κB和ICAM-1的表达。并对大鼠进行神经功能缺损评分。结果NS组、LCIR组、HCIR组大鼠神经功能缺损评分有显著差异（P＜ICAM-1阳性微血管主要表达于缺血周边区，较S组表达明显增强（P＜0.05），LCIR组、HCIR组NF-κB阳性细胞表达率及ICAM-1阳性微血管数较NS组减少（P＜0.05）, LCIR组、HCIR组之间未见明显差异（P＞0.05），每组不同时间点之间NF-κB阳性细胞表达率有明显差异（P＜0.05）以及它们之间的ICAM-1阳性微血管数也有明显差异（P＜0.05）。结论 celecoxib可能通过抑制糖尿病大鼠脑缺血-再灌注后脑组织中NF-κB和ICAM-1的表达，减轻神经功能缺损，从而发挥脑保护作用。 【关键词】 celecoxib 糖尿病 脑缺血再灌注 核因子 细胞间粘附因子 Effects of celecoxib on the expression of NF-κB and ICAM-1 in diabetic rats with focal cerebral ischemia-reperfusion Qi Xue-zhang, Fan Rui-ming, Yang Li, Yu Chang-yin Corresponding Author: Fan Rui-ming,E-mail:erich_333@163.com. 【Abstract】 Objective To investigate the effects of celecoxib on the expressionκB and ICAM-1 in diabetic rats with focal cerebral ischemic reperfusion. Methods Diabetes model was made by intraperitoneal injection of streptozotocin (STZ) and cerebral ischemia reperfusion (IR) model was developed by longe suture occluded method in middle cerebral artery of diabetic rats. The rats were divided into sham operation group(S group), cerebral ischemia-reperfusion in normal saline group (NS group), celecoxib low-dose group (LCIR group) and high-dose group (HCIR group).After 30 minutes following ischemia, rats were respectively given normal saline or celecoxib solution. Respectively at 6h,12h,24h,48h after reperfusion, the rats were decapitated and the brains were collected. The dynamic expression of NF-κB and ICAM-1 in the ipsilateral cortex was detected with immunohistochemistry and the neurological deficit scores were done. Results : the neurological deficit scores are significant difference among the NS, LCIR and HCIR group(P0.05), In the cortex of the rats of NS, LCIR and HCIR