IL-15改善铜绿假单胞杆菌脓毒症小鼠的免疫抑制.docVIP

IL-15改善铜绿假单胞杆菌脓毒症小鼠的免疫抑制 王晓娟，刘德贵，杨 勇，张彦标，薛 刚（610083成都成都军区总医院烧伤整形科） 探讨IL-15是否能够有效纠正脓毒症小鼠的免疫抑制，改善脓毒症小鼠的生存率。：将铜绿假单胞杆菌经尾静脉注射至小鼠构建脓毒症小鼠模型，0.5、1 μg IL-15组注射1 μg IL-15。观察脓毒症小鼠的生存时间，计算脏器细菌定植数量，检测小鼠T细胞和NK细胞的含量，并测定小鼠外周血细胞因子IFN-γ的表达水平。0.5、1 μg IL-15组小鼠生存3.8±3.9）和 (5.0±4.9) d，明显长于对照组[(1.9±0.9) d]。腹腔注射IL-15后，小鼠脾脏细菌的定植量[0.5μg 组：(15.4±2.9)×103 CFU/μg, 1μg 组：(9.7±1.5)×103 CFU/μg]显著低于对照组 [（29.8±3.2）×103CFU/μg, P0.01]。腹腔注射1μgIL-15有效的增加了小鼠T细胞±13.2）% vs （58.6±6.2）%，P0.01]、NK细胞的数量±5.8）% vs （9.8±1.3 %），P0.01]、IFN-γ的表达水平±23.8） vs （289.4±16.2）pg/ml, P0.01]。IL-15能够有效的纠正脓毒症小鼠的免疫抑制，充分活化天然免疫系统和获得性免疫系统，从而改善脓毒症小鼠的生存状况。 IL-15；脓毒症免疫细胞IFN-γ；小鼠Effect of IL-15 on immunosuppression of mice with sepsis caused by Pseudomonas aeruginosa Wang Xiaojuan, Liu Degui, Yang Yong, Zhang Yanbiao, Xue Gang (Department of Burn and Plastic Surgery, General Hospital of Chengdu Military Command, Chengdu, Sichuan Province, 610083,China) [Abstract] Objective To investigate whether IL-15 can efficiently correct the immunosuppression of mice with sepsis, and to improve the survive rate of mice with sepsis. Methods Pseudomonas aeruginosa was injected into C57 mice via the caudal vein to build a mouse model of sepsis. The mice with sepsis were divided into a control group (treated by physiological saline via i.p. injection), a first treatment group (treated by 0.5 μg IL-15 via i.p. injection), and a second treatment group (treated by 1 μg IL-15 via i.p. injection). The survival time of the mice with sepsis was observed. The quantity of bacteria immobilized to the spleens of the mice with sepsis was calculated. The T cell content and NK cell content in mouse blood were analyzed. The expression level of IFN-γ in the peripheral blood of the mice was analyzed. Results The survival time of the mice with sepsis in the control group (1.9±0.9 d) was significantly shorter than those in the first and second treatment groups (3.8±3.9 d and 5.0±4.9 d). The quantity of bacteria immobilized to the spleens of the mice with sepsis in