VEGF,AR在HIFU治疗外阴上皮内非瘤样病变前后的表达及意义.docVIP

VEGF、AR在HIFU治疗外阴上皮内非瘤样病变前后的表达及意义 ，肖松舒2，邓新粮* (1长沙市妇幼保健院，2.3中南大学湘雅三医院,湖南长沙410013) 【摘要】目的：检测高强度聚焦超声(HIFU)治疗外阴上皮内非瘤样病变(NNEDV)前、后血管内皮生长因子(VEGF)与雄激素受体(AR)表达的差异，探讨HIFU治疗NNDEV的作用机制。方法：应用免疫组化检测HIFU治疗NNDEV前、后组织中VEGF、AR蛋白的表达差异；光镜下比较病理学转归。结果： VEGF在病变组织表达高于正常组织(P＜0.0)，治疗后1月治疗前正常组织 (P＜0.0)，治疗后3月表达高于正常组织(P＜0.05)稍低于治疗前但无差异(P＞0.05)AR在病变及正常组织中呈阴性或弱阳性无差异(P＞0.05)治疗后1月与治疗前、正常组织比较亦无差异(P＞0.05)治疗后3月高于治疗前、治疗后1月及正常组织(P＜0.0)；VEGF、AR在不同病理类型的表达无差异(P＞0.05)治疗前表皮呈不同程度的增生或萎缩，大量炎症细胞浸润，微血管减少治疗后1～3个月组织结构逐渐恢复正常，炎症细胞减少，微血管增多。结论：HIFU可有效治疗外阴上皮内非瘤样病变治疗短期内VEGF增加，后期反馈性下降，可能是机制之一皮肤基底层AR表达的增加可能是治疗该病变的机制之一 关键词：高强度聚焦超声，外阴上皮内非瘤样病变，病理改变，血管内皮生长因子，雄激素受体The expressions and significants of vascular endothelial growth factor (VEGF) and androgen receptor (AR) in Nonneoplastic Epithelial Disorders of Vulvar (NNEDV) tissue before and after treated by high-intensity focused ultrasound ( HIFU ) He xiaoli，Xiao songshu，Deng xinliang*, (Dept of Obstetrics and Gynecology of the 3rd Xiangya Hospital of Central South University,Changsha,Hunan 410013 )【Abstract】Objective: To detect the different expressions of vascular endothelial growth factor (VEGF) and androgen receptor (AR) in Nonneoplastic Epithelial Disorders of Vulvar (NNEDV) tissue before and after treated by high-intensity focused ultrasound (HIFU),so that to investigate the mechanism of high-intensity focused ultrasound in managing nonneoplastic epithelial disorders of vulvar. Methods: Adopt imunohistochemistry of SP to detect the different expressions of VEGF and AR in NNEDV tissue before and after treated by HIFU, and compare the pathobiological turnovers by light microscope. Results:1.The expression of VEGF in the lesion tissue group is significantly stronger than that in the normal tissue group(P＜0.01), 1 month after treatment group has the strongest expression of VEGF,and there are significant differences compared with pretherapy group, 3 months after treatment group and normal tissue group(P＜0.01). Furthermore, 3 months after treatment group is significantly stron