大鼠大网膜肝片移植的实验研究.docVIP

大鼠大网膜肝片移植的实验研究 许桂祥① 杜骁① 薛冰川② (山西医科大学第一临床医学院普外科,　太原　030001;　山西医科大学第三医院 太原 030053) 摘　要：目的　研究自体肝片大网膜移植后，肝片生长情况，为肝脏外科临床及实验研究提供理论依据。方法　Wistar 大鼠20只,随机分为4组, 5只大鼠均左外肝叶切除自体肝片大网膜移植，分别于术后3、7、14、21d分批处死动物。肉眼观察移植物颜色、大小及粘连情况，HE法染色,常规光镜观察移植肝片在不同时期的组织学特点。结果　自体肝片移植术后3d 即有血管从移植肝片周围大网膜向肝片内伸展，伴肝细胞凝固性坏死，随时间推移肝片进一步坏死至消失。结论　自体肝片大网膜内移植是简便的肝移植方法，但其存活性有待进一步深入研究。 关键词: 肝脏;自体移植;大鼠 中图分类号：　R657.6;R392　　 Experimental Study on the Livers Patch Transplanted in Epiploon in Rats Xu Guixiang ① Du Xiao ① Xue Bingchuan ② (Dept of General Surgery,First Clinical Medical College,Shanxi Medical University, Taiyuan 030001 ,China; Dept of General Surgery,Third Clinical Medical College,Shanxi Medical University, Taiyuan 030053,China)　　 Abstract : Objective 　To study the growing process of the livers patch in liver auto-transplantaion and to provide data for clinical liver auto-transplantation or liver experimental research. Methods Twenty healthy Wistar rats were equally divided into four groups. In each group ,The ectomy of the left outer liver and liver auto-transplantation were performed in 5 animals ，the animals were killed in batches respectively after 3,7,14,21d . Observe the color, size and adhesion of each patch through the naked eye，HE staining，Observe the histological features of the livers patches transplanted at different times under Conventional light microscopy. Results 　Three days after operation , it was found that the renascent blood vessels were extended from epiploon into patches with coagulative necrosis of liver cells. With the time going on ，the become further necrosis to disappear. Conclusion 　Autotransplantation of liver into epiploon is a convenient way for liver transplantation，but its viability remains to be further study. Key words Liver ；Autotransplantation；Rats 含肝素25IU/ml的4度乳酸林格液10 ml 每日肌注青霉素8万单位/只 0 等大 红白相间 4 B 5 25 0 变小 乳白 2 C 5 25 0 更小 黄白