quantitative resistance of potato to pectobacterium atrosepticum and phytophthora infestans integrating pamp-triggered response and pathogen growth定量阻力土豆pectobacterium atrosepticum和5种整合pamp-triggered响应和病原体的生长.pdfVIP

Quantitative Resistance of Potato to Pectobacterium atrosepticum and Phytophthora infestans: Integrating PAMP-Triggered Response and Pathogen Growth ¨ ¨ Alexander Kroner, Gaelle Hamelin, Didier Andrivon, Florence Val* ´ INRA (French National Institute for Agricultural Research), Agrocampus Ouest, Universite de Rennes1, UMR (Mixed Research Unit) 1099 BiO3P (Biology of Organisms and Populations applied to Plant Protection), Rennes, France Abstract While the mechanisms underlying quantitative resistance of plants to pathogens are still not fully elucidated, the Pathogen- Associated Molecular Patterns (PAMPs)-triggered response model suggests that such resistance depends on a dynamic interplay between the plant and the pathogen. In this model, the pathogens themselves or elicitors they produce would induce general defense pathways, which in turn limit pathogen growth and host colonisation. It therefore suggests that quantitative resistance is directly linked to a common set of general host defense mechanisms, but experimental evidence is still inconclusive. We tested the PAMP-triggered model using two pathogens (Pectobacterium atrosepticum and Phytophthora infestans) differing by their infectious processes and five potato cultivars spanning a range of resistance levels to each pathogen. Phenylalanine ammonia-lyase (PAL) activity, used as a defense marker, and accumulation of phenolics were measured in tuber slices challenged with lipopolysaccharides from P. atrosepticum or a concentrated culture filtrate from P. infestans. PAL activity increased following treatment with the filtrate but not with lipopolysaccharides, and varied among cultivars. It was positi