quantitative analysis of peripheral tissue perfusion using spatiotemporal molecular dynamics定量分析的外围组织灌注使用时空分子动力学.pdfVIP

Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics 1. 1. 1 2,3,4 5 Yujung Kang , Myunghwan Choi , Jungsul Lee , Gou Young Koh , Kihwan Kwon , Chulhee Choi1,3,4* 1 Department of Bio and Brain Engineering, KAIST, Daejeon, Korea, 2 Department of Biological Sciences, KAIST, Daejeon, Korea, 3 Graduate School of Medical Science and Engineering, KAIST, Daejeon, Korea, 4 KI for the BioCentury, KAIST, Daejeon, Korea, 5 Department of Cardiology, School of Medicine, Ewha Womans University, Seoul, Korea Abstract Background: Accurate measurement of peripheral tissue perfusion is challenging but necessary to diagnose peripheral vascular insufficiency. Because near infrared (NIR) radiation can penetrate relatively deep into tissue, significant attention has been given to intravital NIR fluorescence imaging. Methodology/Principal Findings: We developed a new optical imaging-based strategy for quantitative measurement of peripheral tissue perfusion by time-series analysis of local pharmacokinetics of the NIR fluorophore, indocyanine green (ICG). Time-series NIR fluorescence images were obtained after injecting ICG intravenously in a murine hindlimb ischemia model. Mathematical modeling and computational simulations were used for translating time-series ICG images into quantitative pixel perfusion rates and a perfusion map. We could successfully predict the prognosis of ischemic hindlimbs based on the perfusion profiles obtained immediately after surgery, which were dependent on the preexisting collaterals. This method also reflected increases in perfusion and improvements in prognosis of ischemic hindlimbs induced by treatment with vascular endothelial growth