quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons定量和定性茎锈病抗性因素大麦与国防转录抑制调节子相关联.pdfVIP

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quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons定量和定性茎锈病抗性因素大麦与国防转录抑制调节子相关联.pdf

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quantitative and qualitative stem rust resistance factors in barley are associated with transcriptional suppression of defense regulons定量和定性茎锈病抗性因素大麦与国防转录抑制调节子相关联

Quantitative and Qualitative Stem Rust Resistance Factors in Barley Are Associated with Transcriptional Suppression of Defense Regulons 1,2,3¤ 2,4 5 1,2,3,4 Matthew J. Moscou , Nick Lauter *, Brian Steffenson , Roger P. Wise * 1 Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, Iowa, United States of America, 2 Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa, United States of America, 3 Center for Responses to Environmental Stresses, Iowa State University, Ames, Iowa, United States of America, 4 Corn Insects and Crop Genetics Research, Agricultural Research Service, United States Department of Agriculture, Iowa State University, Ames, Iowa, United States of America, 5 Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota, United States of America Abstract Stem rust (Puccinia graminis f. sp. tritici; Pgt) is a devastating fungal disease of wheat and barley. Pgt race TTKSK (isolate Ug99) is a serious threat to these Triticeae grain crops because resistance is rare. In barley, the complex Rpg-TTKSK locus on chromosome 5H is presently the only known source of qualitative resistance to this aggressive Pgt race. Segregation for resistance observed on seedlings of the Q21861 6SM89010 (QSM) doubled-haploid (DH) population was found to be predominantly qualitative, with little of the remaining variance explained by loci other than Rpg-TTKSK. In contrast, analysis of adult QSM DH plants infected by field inoculum of Pgt race TTKSK in Njoro, Kenya, revealed several additional quantitative trait loci that contribute to resistance. To molecularly characterize these loci, Barley1 GeneChip