quantitative analysis of genetic and neuronal multi-perturbation experiments定量分析的遗传和神经multi-perturbation实验.pdfVIP

Quantitative Analysis of Genetic and Neuronal Multi-Perturbation Experiments 1[* 2[ 3 4 2,5* Alon Kaufman , Alon Keinan , Isaac Meilijson , Martin Kupiec , Eytan Ruppin 1 Interdisciplinary Center for Neural Computation, Hebrew University, Jerusalem, Israel, 2 School of Computer Science, Tel-Aviv University, Tel-Aviv, Israel, 3 School of Mathematical Sciences, Tel-Aviv University, Tel-Aviv, Israel, 4 Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Tel-Aviv, Israel, 5 School of Medicine, Tel-Aviv University, Tel-Aviv, Israel Perturbation studies, in which functional performance is measured after deletion, mutation, or lesion of elements of a biological system, have been traditionally employed in many fields in biology. The vast majority of these studies have been qualitative and have employed single perturbations, often resulting in little phenotypic effect. Recently, newly emerging experimental techniques have allowed researchers to carry out concomitant multi-perturbations and to uncover the causal functional contributions of system elements. This study presents a rigorous and quantitative multi- perturbation analysis of gene knockout and neuronal ablation experiments. In both cases, a quantification of the elements’ contributions, and new insights and predictions, are provided. Multi-perturbation analysis has a potentially wide range of applications and is gradually becoming an essential tool in biology. Citation: Kaufman A, Keinan A, Meilijson I, Kupiec M, Ruppin E (2005) Quantitative analysis of genetic and neuronal multi-perturbation experiments. PLoS Comput Biol 1(6): e64. Introduction To address this challenge, Keinan et al. [16] developed the