recombine a suite of programs for detection and analysis of meiotic recombination in whole-genome datasets重组一套项目检测和分析全基因组数据集的减数分裂重组.pdfVIP

ReCombine: A Suite of Programs for Detection and Analysis of Meiotic Recombination in Whole-Genome Datasets 1 1 2,3 1 2,4 Carol M. Anderson , Stacy Y. Chen , Michelle T. Dimon , Ashwini Oke , Joseph L. DeRisi , Jennifer C. Fung1* 1 Department of Obstetrics, Gynecology, and Reproductive Sciences and Center for Reproductive Sciences, University of California San Francisco, San Francisco, California, United States of America, 2 Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America, 3 Biological and Medical Informatics Program, University of California San Francisco, San Francisco, California, United States of America, 4 Howard Hughes Medical Institute, Bethesda, Maryland, United States of America Abstract In meiosis, the exchange of DNA between chromosomes by homologous recombination is a critical step that ensures proper chromosome segregation and increases genetic diversity. Products of recombination include reciprocal exchanges, known as crossovers, and non-reciprocal gene conversions or non-crossovers. The mechanisms underlying meiotic recombination remain elusive, largely because of the difficulty of analyzing large numbers of recombination events by traditional genetic methods. These traditional methods are increasingly being superseded by high-throughput techniques capable of surveying meiotic recombination on a genome-wide basis. Next-generation sequencing or microarray hybridization is used to genotype thousands of polymorphic markers in the progeny of