rana grylio virus (rgv) 50l is associated with viral matrix and exhibited two distribution patternsrana grylio病毒(rgv)50 l与病毒相关矩阵和表现出两种分配模式.pdfVIP

Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns Xiao-Ying Lei, Tong Ou, Qi-Ya Zhang* State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan, China Abstract Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet. Methodology/Principal Findings: We cloned and characterized RGV50L, and revealed 50L functions in virus assembly and gene regulation. 50L encoded a 499-amino acid structural protein of about 85 kDa in molecular weight and contained a nuclear localization signal (NLS) and a helix- extension-helix motif. Drug inhibition assay demonstrated that 50L was an immediate-early (IE) gene. Immuno-fluorescence assay revealed that 50L appeared early and persisted in RGV-infected cells following two distribution patterns. One pattern was that 50L exhibited a cytoplasm-nucleus- viromatrix distribution pattern, and mutagenesis of the NLS motif revealed that localization of 50L in the nucleus was NLS-dependent; the other was that 50L co-localized with viral matrix which plays important roles in virus assembly and the life circle of viruses. Conclusions/Significance: RGV 50L is a novel iridovirus IE gene encoded structural protein which plays important roles in virus assembly. Citation: Lei X-Y, Ou T, Zhang Q-Y (2012) Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns. PLoS ONE 7(8): e43033. doi:10.1371/journal.pone.0043033 Editor: Jianming Qiu, University of Kansas Medical Center, United States of America Received April 11, 20