原创力文档rhamnose-inducible gene expression in listeria monocytogenes单核细胞增多性李斯特氏菌rhamnose-inducible基因表达.pdfVIP
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rhamnose-inducible gene expression in listeria monocytogenes单核细胞增多性李斯特氏菌rhamnose-inducible基因表达
Rhamnose-Inducible Gene Expression in Listeria
monocytogenes
Lars Fieseler.¤a, Sibylle Schmitter., Justinas Teiserskas¤b, Martin J. Loessner*
Institute of Food, Nutrition, and Health, ETH Zurich, Zurich, Switzerland
Abstract
Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the
rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter
Prha. Expression of reporter genes under control of Prha on a single copy integration vector demonstrated its suitability for
inducible gene expression in L. monocytogenes. Transcription initiation from Prha is dose dependent, and a concentration as
low as 100 mM rhamnose was found sufficient for induction. Moreover, Prha is subject to glucose catabolite repression,
which provides additional options for strict control of expression. Infection of human THP1 macrophages revealed that Prha
is repressed in intracellular L. monocytogenes, which is explained by the absence of rhamnose in the cytosol and possible
interference by catabolite repression. The Prha promoter provides a novel and useful tool for triggering gene expression in
extracellular L. monocytogenes, whereas intracellular conditions prevent transcription from this promoter.
Citation: Fieseler L, Schmitter S, Teiserskas J, Loessner MJ (2012) Rhamnose-Inducible Gene Expression in Listeria monocytogenes. PLoS ONE 7(8): e43444.
doi:10.1371/journal.pone.0043444
Editor: Nancy E. Freitag, University of Illinois at Chicago College of Medicine, United States of America
Received April 26, 2012; Accepted July 20, 2012; Published August 22, 2012
Copyright: 2012 Fieseler et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
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