rho kinases role in myosin recruitment to the equatorial cortex of mitotic drosophila s2 cells is for myosin regulatory light chain phosphorylationρ在肌凝蛋白激酶的作用招聘的赤道皮层细胞有丝分裂果蝇s2是肌球蛋白轻链磷酸化监管.pdfVIP

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rho kinases role in myosin recruitment to the equatorial cortex of mitotic drosophila s2 cells is for myosin regulatory light chain phosphorylationρ在肌凝蛋白激酶的作用招聘的赤道皮层细胞有丝分裂果蝇s2是肌球蛋白轻链磷酸化监管.pdf

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rho kinases role in myosin recruitment to the equatorial cortex of mitotic drosophila s2 cells is for myosin regulatory light chain phosphorylationρ在肌凝蛋白激酶的作用招聘的赤道皮层细胞有丝分裂果蝇s2是肌球蛋白轻链磷酸化监管

Rho Kinase’s Role in Myosin Recruitment to the Equatorial Cortex of Mitotic Drosophila S2 Cells Is for Myosin Regulatory Light Chain Phosphorylation Sara O. Dean, James A. Spudich* Department of Biochemistry, Stanford University, Stanford, California, United States of America Background. Myosin II recruitment to the equatorial cortex is one of the earliest events in establishment of the cytokinetic contractile ring. In Drosophila S2 cells, we previously showed that myosin II is recruited to the furrow independently of F-actin, and that Rho1 and Rok are essential for this recruitment [1]. Rok phosphorylates several cellular proteins, including the myosin regulatory light chain (RLC). Methodology/Principal Findings. Here we express phosphorylation state mimic constructs of the RLC in S2 cells to examine the role of RLC phosphorylation involving Rok in the localization of myosin. Phosphorylation of the RLC is required for myosin localization to the equatorial cortex during mitosis, and the essential role of Rok in this localization and for cytokinesis is to maintain phosphorylation of the RLC. The ability to regulate the RLC phosphorylation state spatio-temporally is not essential for the myosin localization. Furthermore, the essential role of Citron in cytokinesis is not phosphorylation of the RLC. Conclusions/Significance. We conclude that the Rho1 pathway leading to myosin localization to the future cytokinetic furrow is relatively straightforward, where only Rok is needed, and it is only needed to maintain phosphorylation of the myosin RLC. Citation: Dean SO, Spudich JA (2006) Rho Kinase’s Role in Myosin Recruitment to the Equatorial Cortex of Mitotic Drosophila S2 Cells Is for Myosin Regulatory Light Chain Phosphorylation. PLoS ONE 1(1): e131. doi:10.1371/journal.pone.0000131 INTRODUCTION Drosophila embryos in mutants o