sensing of dietary lipids by enterocytes a new role for sr-bicla-1传感的膳食脂质为sr-bicla-1肠上皮细胞一个新的角色.pdfVIP

Sensing of Dietary Lipids by Enterocytes: A New Role for SR-BI/CLA-1 ´ 1,2,3 1,2,3 1,2,3 1,2,3 1,2,3 Olivier Beaslas , Carine Cueille , Franc¸ois Delers , Danielle Chateau , Jean Chambaz , 1,2,3 ´ ` 1,2,3 Monique Rousset , Veronique Carriere * ´ ´ 1 Universite Pierre et Marie Curie - Paris 6, UMR S 872, Les Cordeliers, Paris, France, 2 INSERM, U 872, Paris, France, 3 Universite Paris Descartes, UMR S 872, Paris, France Abstract Background: The intestine is responsible for absorbing dietary lipids and delivering them to the organism as triglyceride- rich lipoproteins (TRL). It is important to determine how this process is regulated in enterocytes, the absorptive cells of the intestine, as prolonged postprandial hypertriglyceridemia is a known risk factor for atherosclerosis. During the postprandial period, dietary lipids, mostly triglycerides (TG) hydrolyzed by pancreatic enzymes, are combined with bile products and reach the apical membrane of enterocytes as postprandial micelles (PPM). Our aim was to determine whether these micelles induce, in enterocytes, specific early cell signaling events that could control the processes leading to TRL secretion. Methodology/Principal Findings: The effects of supplying PPM to the apex of Caco-2/TC7 enterocytes were analyzed. Micelles devoid of TG hydrolysis products, like those present in the intestinal lumen in the interprandial period, were used as controls. The apical delivery of PPM