il-1与创伤性脑水肿的关系（Relationship between IL-1 and traumatic brain edema）.docVIP

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il-1与创伤性脑水肿的关系（Relationship between IL-1 and traumatic brain edema）.doc

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il-1与创伤性脑水肿的关系（Relationship between IL-1 and traumatic brain edema）

il-1与创伤性脑水肿的关系（Relationship between IL-1 and traumatic brain edema） The relationship between IL-1 and traumatic brain edema Update Date: 2011-09-15, Jiang poly, Hong Zhongping, Xu Qiwu A number of studies have shown that interleukin 1 (IL 1) of the secondary brain edema in trauma, therefore the use of interleukin 1 receptor antagonist (IL - 1Ra) for the treatment of traumatic brain edema has a good application prospect, 1, 5. By using radioimmunoassay and dot blot hybridization method, the expression of the injured brain edema animal model of traumatic brain water content, IL 1 beta and mRNA determination, to investigate the efficacy of IL 1 with traumatic brain edema and the relationship between IL and 1Ra in treatment of traumatic brain edema. Materials and methods 1. animal and group: 180 male Wistar rats, weight (255 + 29.8) g, were randomly divided into normal group, sham injury group, injury group and IL-1ra group, the latter is based on the use of antagonists of time into injury 2 hours before and 2 hours after injury group. 2. brain trauma model and material: trauma models were Feeney method [1], the injury impulse 600g.cm, the injury area of 12.6 mm2. Is the injection method Yamasaki method 2. The successful animal models were sacrificed at 4, 24 and 72 hours after injury, and sacrificed by different groups. The water content of brain tissue (wet and dry weight), IL 1 beta and total mRNA were extracted. The whole brain was immediately placed in a Formaldehyde Solution with a volume fraction of 10%. Paraffin embedded sections were taken and examined by light microscopy. 3. brain IL-1 beta and radioimmunoassay extraction: the use of the Chinese Academy of Sciences Institute of basic medicine pharmacology of IL-1 beta RIA kit, according to the kit instructions. 4. dot blot: total mRNA was extracted by guanidine thiocyanate was preserved in liquid nitrogen. IL - 1 beta cDNA plasmid was amplified by PCR, linearized with restriction enzyme, labeled with SP6 vecto