几丁聚糖对人脐静脉内皮细胞凋亡及相关基因表达影响.pdfVIP

结论 一定浓度的几丁聚糖可以增加人脐静脉内皮细胞Bcl-2基因的表达 产物,同时降低Bax 基因的表达产物，降低凋亡率，并可增加内皮细胞代谢活性。 关键词 几丁聚糖 人脐静脉内皮细胞 凋亡 4 Astudy forchitosan toinfluence theexpression ofapoptosic geneandapoptosisin hum [OBJECTIVE] of apoptosic gene in human u find a new stent coating substance . [METHODS] We putChitosan with different c ① group(Chi 0mg/ml)② Chi:0.01mg/ml③Chi:0.1mg/mg④ activity bymethylthiazolyltetrazolium (MTT)、endothelial cell methylthiazolyltetrazolium apoptosic rate by FCM、the Bcl-2 and Immunocytochemcal(IHC)、cell apoptosic modality by AO、the expression of Bcl- reaction (RT-PCR). [RESULTS]⑴ increased by MTT as Chi concertrationincreased increased,and ⑵The HUVECs 、 20.01+2.7%、 38.1±3.3%）;the difference while the difference.by FCM⑶ Chi could significantly increased Bcl- protein expression as its concentration（Chi：0.01mg/ml～1 mg/ml） increased by Immunocytochemcal(IHC).⑷ could significantly increased Bcl- 5 protein and Bax mRNA expression reaction . [CONCLUSION] Chi could obviously increased the activity of endothelial cells and decreased apoptosic rate as its concentration,Chi could significantly increased Bcl-2 mRNA and Bcl-2 protein expression and decreased Bax protein and Bax mRNA expression . Postgraduate LI Tie Tutor TAN Xiao-jin Professor The First Affiliated Hospital of Nanhua University Key word: chitosan apoptosis human umbil