ptdTomato-N1哺乳动物表达载体说明.pdfVIP

ptdTomato-N1 编号 载体名称 北京华越洋生物VECT6008 ptdTomato-N1 ptdTomato-N1 载体基本信息 载体名称: ptdTomato-N1 质粒类型: 哺乳动物细胞表达载体；荧光报告载体 高拷贝/低拷贝: 高拷贝 克隆方法: 限制性内切酶，多克隆位点 启动子: CMV IE 载体大小: 5443 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: tdTomato (C-端） 载体抗性: 卡那霉素 筛选标记: 新霉素（Neomycin） 克隆菌株: DH5α, HB101 宿主细胞（系）: 常规细胞系（293、CV-1、CHO 等） 哺乳动物载体ptdTomato-N1 组成型表达C 端pAsRed2 融 备注: 合蛋白。 稳定性: 稳表达 或 瞬表达 组成型/诱导型: 组成型 病毒/非病毒: 非病毒 ptdTomato-N1 载体质粒图谱和多克隆位点信息 ptdTomato-N1 载体描述 ptdTomato-N1 is a mammalian expression vector designed to express a protein of interest fused to the N-terminus of tdTomato. td Tomato is a member of the family of fruit fluorescent proteins (1) derived from the Discosoma sp. red fluorescent protein, DsRed (2). Because the Tomato protein has a tendency to dimerize, the vector was designed with two copies of the Tomato coding region linked together to allow intramolecular dimerization. As a result, each tdTomato RNA transcript encodes a tandem dimer of the Tomato protein (excitation and emission maxima equal 554nm and 581nm, respectively). Expression of td Tomato as a tandem dimer prevents the fused protein of interest from being forced into a dimeric complex. Fusions that retain the fluorescence properties of tdTomato can be monitored by flow cytometry and localized by fluorescence microscopy. The multiple cloning site (MCS) in ptdTomato-N1 is positioned upstream of the tdTomato coding se