vip 2012 小鼠孤雄单倍体胚胎干细胞系首次建立Generation of Genetically Modified Mi.pdfVIP

Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells Hui Yang,1,6 Linyu Shi,1,6 Bang-An Wang,2,6 Dan Liang,1 Cuiqing Zhong,1 Wei Liu,2 Yongzhan Nie,4 Jie Liu,4 Jing Zhao,5 Xiang Gao,5 Dangsheng Li,3 Guo-Liang Xu,2,* and Jinsong Li1,* 1Group of Epigenetic Reprogramming, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology 2Group of DNA Metabolism, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology 3Shanghai Information Center for Life Sciences Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 4State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China 5MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing 210061, China 6These authors contributed equally to this work *Correspondence: glxu@ (G.-L.X.), jsli@ (J.L.) DOI 10.1016/j.cell.2012.04.002 SUMMARY 1978; Latham et al., 2002; Modlinski, 1975; Tarkowski and Ros- sant, 1976), embryonic stem cells (ESCs) established from these Haploid cells are amenable for genetic analysis. haploid embryos have turned out to display a diploid karyotype Recent success in the derivation of mouse haploid (Kaufman et al., 1983). In humans, near-haploid cell lines have embryonic stem cells (haESCs) via parthenogenesis been established from tumors (Carette et al., 2009; Kotecki has enabled genetic screening in mammalian cells. et al., 1999; Sukov et a