DNA分子标记技术在花生品种鉴定上的应用研究_王传堂.pdfVIP

华北农学报·2006, 21(增刊):110-113 DNA 1 1 2 1 1 1 王传堂,杨新道, 于　翔, 张建成,刘光臻 ,徐建芝 (1., 　266100　2., 　610064) 　　:10 , AFL, SRAP, SSR STS 。 ,AFLP SRAP , , , , ; 103 SSR STS 9 , Lec_1,Ah4_26, Ah4_4, SsS14, SHPAL_1, PG_71, PG_43, PM_36, PG_22, 10 100%。、, 9 SSR STS 。 :;; :S565.2　　:A　　:1000-7091(2006)-0110-04 Utilization of DNA Molecular Marker Technology in the Discrimination of Peanut Cultivars 1 1 2 1 1 1 WANG Chuan_tan , YANG Xin_dao , YU Xian , ZHANG Jian_chen , LIU Guan _zhen ,XU Jian_zhi (1.Shandon Peanut Research Institute, Qin dao　266100, China; 2.Sichuan University, Chen du 　610064, China ) Abstract:Ten peanut cultivars from China were utilized in an attempt to evaluate the discrimination power of differ- ent molecular marker systems,includin AFLP,SRAP,SSR and STS.AFLP and SRAP technolo y failed to identify indi- vidual peanut cultivar usin a sin le primer pair, su estin that more primer pairs and/or restriction enzyme combina- tions are needed.Nine primer pairs, viz, Lec_1, Ah4_26,Ah4_4, SsS14,SHPAL_1,PG_71,PG_43,PM_36, PG_22, re- sultin from screenin of 103 SSR/STS primer pairs proved to have the discrimination power of 100%.Owin to the lim- ited number of morpholo ical, cyto enetic and biochemical markers in cultivated peanut, the 9 informative primer pairs are of si nificance in peanut enetic and variety identification studies. Key words:Peanut;Variety identification;Molecular marker 　　30 , 、, , 40 , [1～11] 。 ,、。 SRAP,AFLP,SSR,STS DNA DNA ,、 、、、 。 。 1　材料和方法 DNA RFLP