ZAP-70在24例B细胞慢性淋巴细胞白血病中表达探究.docVIP

ZAP-70在24例B细胞慢性淋巴细胞白血病中表达探究 　 作者：吴雨洁，李建勇，朱光荣，宋君红，肖冰 【摘要】 为了研究慢性淋巴细胞白血病中zeta链相关蛋白-70（zeta-associated protein-70，ZAP-70）的表达及其与其他预后因素的相关性，应用四色流式细胞术检测24例B细胞慢性淋巴细胞白血病(B-CLL)患者骨髓或外周血白血病细胞ZAP-70和CD38的表达。 结果显示：① 37.5% B-CLL患者表达ZAP-70，其中Binet A期患者有20%（3/15）表达ZAP-70+，Binet B+C期患者有66.7%（6/9）表达 ZAP-70+，ZAP-70+表达在Binet A期和Binet B+C期之间有显著差异（Plt;0.05）；② 29.1% B-CLL患者表达CD38，其中Binet A期患者有3例；在此3例中2例同时伴有ZAP-70+，CD38+在Binet A期和Binet B+C期之间无显著差异（Pgt;0.05）；③83.3%（20/24）患者表达ZAP-70+ CD38+ 或ZAP-70-CD38-，ZAP-70和CD38存在相关性（Plt;0.05）。 结论：在常规实验室可以采用流式细胞术检测ZAP-70，ZAP-70高表达与B-CLL临床分期、染色体及CD38表达等预后相关因素有一定的相关性。 【关键词】 慢性淋巴细胞白血病 　　ZAP-70 Expression in 24 Patients with B Cell Chronic Lymphocytic Leukemia 　　Abstract To explore the ZAP-70 expression in chronic lymphocytic leukaemia (CLL) and its relationship with other prognostic factors，the expressions of ZAP-70 protein and CD38 in bone marrow or peripheral blood of 24 patients with B-CLL were determined by four-color flow cytometry.The results showed that ZAP-70 was positively expressed in 37.5% of all B-CLL patients，20% (3/15) patients in Binet A stage，and 66.7% in Binet B+C.The expression of ZAP-70 had significant difference between Binet A and Binet B+C (Plt;0.05).CD38 was expressed in 29.1% of B-CLL patients and 3 out of these cases were in stage A，2 out of 3 cases in stage A co-expressed CD38 and ZAP-70.The expression of CD38 had no significant difference between Binet A and Binet B+C stage (Pgt;0.05).ZAP-70+ and CD38+ were both expressed in 83.3% of B-CLL patients (Plt;0.05).It is concluded that ZAP-70 protein can be routinely measured by flow cytometry in the laboratory.High expression of ZAP-70 is correlated with other prognostic factors such as clinic stage，chromosome abnormalities and CD38 in B-CLL. 　　Key words chronic lymphocytic leukemia; ZAP-70; CD38 Zeta链相关蛋白-70(zeta chain associated protein-70，ZAP-70)是一种与TCRζ相联系的70 kDa蛋白酪氨酸激酶（PTK），广泛表达在T细胞和NK细胞中，在TCR受刺激后出现可诱导的双磷酸化而激活T细胞信号传导通路。正常B淋巴细胞缺乏ZAP-70，在B细胞的信号传导中，另一种PTK-Syk通过B细胞抗原受体（BCR）复合物起着和ZA