Survivin蛋白在小鼠生精恢复过程中表达及其及生殖细胞凋亡关.docVIP

Survivin蛋白在小鼠生精恢复过程中表达及其及生殖细胞凋亡关 　【关键词】 蛋白 　　Expression of survivin proteins in recovery of mice spermatogenesis and its correlation with germ cell apoptosis HU ChuanYi 　　【Abstract】 AIM: To explore the expression of antiapoptotic proteins survivin in the testis tissue and its correlation with germ cells apoptosis. METHODS: Adult Kunming mice were injected intraperitoneally with two doses of busulfan at 10 mg/kg bodyweight at an interval of 24 h, so as to establish the models in the process of spermatogenesis recovery. Testes were harvested in 1, 2, 3, 4, 6, 8, 10 weeks after the second injection. The survivin expression was detected by immunohistochemistry SP method and the germ cell apoptosis was detected by transmission electron microscope and terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL) technique. RESULTS: Survivin was mostly expressed in the leydig cells, secondary spermatocytes and spermatoblasts. The germ cell apoptosis mainly occurred in primary spermatocytes and round spermatoblasts. Survivin expression was negatively correlated with sperm cell apoptosis (r=-0.784, Plt;0.01). CONCLUSION: Survivininduced inhibition of apoptosis may participate in the balance of apoptosis and thus plays an important role in the normal spermatogenesis. 　　【Keywords】 survivin protein; apoptosis; germ cells 　　【摘要】 目的: 探讨新的凋亡相关基因survivin蛋白在小鼠生精恢复过程中的表达及其与生殖细胞凋亡的关系. 方法: 间隔24 d两次腹腔注射白消安建立小鼠生精恢复过程的动物模型，第2次给药后随机分为1，2，3，4，6，8，10 wk 7组，于相应时间点及给药前取材，采用免疫组化SP法和透射电镜、DNA原位末端标记TUNEL法分别检测survivin蛋白的表达和细胞凋亡. 结果: Survivin蛋白主要表达于间质细胞、次级精母细胞和精子细胞，生殖细胞的凋亡主要发生于初级精母细胞和圆头精子细胞. Survivin蛋白的表达与生殖细胞凋亡指数（AI）呈负相关(r=-0.784，Plt;0.01). 结论: Survivin蛋白表达而引起的凋亡抑制，有利于生殖细胞凋亡稳态的维持，对正常的精子发生起重要作用. 　　【关键词】 survivin蛋白；凋亡；生殖细胞 　　0引言 　　精子发生涉及生殖细胞（germ cells, Gc）的增殖和自发性凋亡等过程，在正常的精子发生过程中，Gc的增殖和凋亡保持一定的平衡状态. Survivin是近年来分离出的凋亡抑制基因家族中的新成员，在调控细胞周期和抑制细胞凋亡中可能起重要作用. 我们通过对survivin蛋白在小鼠生精恢复过程中的表达变化规律及其与Gc凋亡的关系，试图阐明survivin对小鼠Gc凋亡的调控作用，以期能为男性生育的临床研究提供理论及动物实验的依据. 　　1材料和方法 　　1.1动物模型的建立和分组昆明雄性2~3 mo龄小白鼠56