PLK-1在急性白血病细胞中表达及意义.docVIP

PLK-1在急性白血病细胞中表达及意义 　 作者：毛汉文，刘文励，周剑峰，孙汉英，徐惠珍，罗小华 【摘要】 为了探讨急性白血病细胞中plk-1基因及其蛋白的表达情况和意义，并初步了解PLK-1蛋白在急性白血病细胞中的分布情况，通过抽取急性白血病患者、骨髓良性增生者及正常人的骨髓或外周血单个核细胞作为研究对象,应用RT-PCR技术及流式细胞术检测plk-1 mRNA和PLK-1蛋白在急性白血病细胞中的表达，用荧光显微镜观察PLK-1蛋白在急性白血病细胞中的分布。结果表明：急性白血病细胞plk-1在基因水平及蛋白质水平上表达均明显增高；荧光显微镜检查显示细胞分裂间期PLK-1蛋白较高浓度地、均匀地散在分布于急性白血病细胞内，在有丝分裂过程中，姊妹染色单体拉开至细胞两极时PLK-1蛋白主要分布在姊妹染色单体之间。而正常骨髓细胞和良性增生骨髓细胞plk-1在基因水平和蛋白质水平几乎均不表达。结论： PLK-1蛋白对急性白血病细胞的异常增殖可能起重要作用，其表达的高低与恶性程度有关。PLK-1蛋白或其基因有可能作为抗瘤药物新的靶点，并可作为判断急性白血病疗效及预后的有效指标之一。 【关键词】 急性白血病； plk-1基因； PLK-1蛋白；急性白血病细胞； 基因表达 　　Expression of plk-1 Gene in Acute Leukemia Patients and Its Significanc 　　AbstractTo investigate expression of plk-1 gene and PLK-1 protein in acute leukemia patients and its clinical significance, and to observe the distribution of PLk-1 protein in acute leukemin cells, the mononuclear cells were separated from the bone marrow or peripheral blood of acute leukemia patients, bone marrow benign proliferation individuals and normal individuals. The expression of plk-1 gene and PLK-1 protein in those cells were detected with RT-PCR and flow cytometry respectively, the distribution pattern of PLK-1 was observed by fluorescent inverted microscope. The result showed that the expressions of plk-1 gene and PLK-1 protein in mononuclear cells of acute leukemia patients were much higher than that of bone marrow benign proliferation individuals and normal individuals. Fluorescent inverted microscopy revealed that PLK-1 was highly concentrated in cytoplasm of acute leukemia cells during interphase of mitosis, and it was found that PLK-1 was mainly distributed between sister chromatid during the mitosis in mononuclear cells of acute leukemia patients, but the expressions of plk-1 gene and PLK-1 protein almost were not observed in cells of benign proliferative bone marrow and normal bone marrow. It is concluded that increased plk-1 gene and protein perhaps play an important role in abnormal proliferation of acute leukemia cells and correlate with the