不同治法中药复方对H22肝癌小鼠瘤质量及体质量及免疫功能影响.docVIP

不同治法中药复方对H22肝癌小鼠瘤质量及体质量及免疫功能影响 　作者：周阿高, 张勇, 蒉纲, 孔德云, 葛海良 任秋华, 董佳容, 洪声, 毛旭明, 王颖, 张惠珍, 王树军 【摘要】 比较不同治法的中药复方对H22肝癌小鼠瘤质量、体质量及免疫功能等的影响。方法：选择移植性肝癌H22小鼠为模型，观察不同治法的中药复方常规水煎剂的抑瘤作用，并通过检测各组小鼠T淋巴细胞增殖功能、自然杀伤细胞（natural killer, NK）杀伤功能、T淋巴细胞表型变化及γ干扰素（interferon-γ, IFN-γ）和白细胞介素-4（interleukin-4, IL-4）含量来比较免疫功能状况。结果：以毒攻毒组小鼠瘤质量显著小于其他各组小鼠，抑瘤率达65.8％（Plt;0.01）；其他4组抑瘤率在10.1％～17.1％之间。以毒攻毒组小鼠体质量明显下降，出现脱毛现象；扶正培本组小鼠毛发生长良好，活泼好动。以毒攻毒组小鼠T淋巴细胞转化刺激指数（stimulation index, SI）增加显著（SI=4.34，Plt;0.01），说明以毒攻毒组小鼠T细胞具有较强的增殖能力；其余各组小鼠SI均低于3，表明这些组的小鼠T细胞无明显增殖作用。清热解毒、活血化瘀和以毒攻毒组小鼠NK细胞百分数显著增高，分别为生理盐水组的5.05、4.07和5.17倍（Plt;0.01）；扶正培本和化痰散结组小鼠NK细胞杀伤活性未见显著升高。IFN-γ检测结果显示，以毒攻毒组小鼠T细胞分泌IFN-γ能力显著升高（Plt;0.05）；IL-4检测结果显示，除扶正培本组外，清热解毒、活血化瘀、化痰散结和以毒攻毒组小鼠T细胞分泌IL-4的能力也均显著升高（Plt;0.01）。结论：以毒攻毒法能有效抑制肿瘤细胞的生长，明显增强小鼠细胞和体液免疫功能；扶正培本法则有良好的强壮作用。 【关键词】 中医治法; 中药复方; 肝癌;体质量; 免疫力; 小鼠 Objective: To compare the influence of traditional Chinese compound recipes (TCCRs) with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Methods: H22 cancer-bearing mice were chosen to observe the effects of TCCRs with different efficacy on tumor growth inhibition and detect the proliferation function of T lymphocytes, the activity of natural killer (NK) cells, the changes of T lymphocytes and the content of interferon-γ (IFN-γ）and interleukin-4 (IL-4). Results: Tumor weight of H22 cancer-bearing mice in Yidu Gongdu Recipe (YDGDR, a compound traditional Chinese herbal medicine using poison as an antidote for poison)-treated group was obviously lighter than that in the other TCCR-treated groups and the tumor inhibition rate in YDGDR-treated group was 65.76% (Plt;0.01). The tumor inhibition rates in other TCCR-treated groups were ranged from 10.1％ to 17.1%. Body weight of mice in YDGDR-treated group was obviously decreased and depilation was observed at the same time. Pelage of mice in Fuzheng Peiben Recipe (FZPBR, a compound traditional Chinese herbal medicine for supporting the healthy energy)-treated group grew we