人类宫颈癌中白细胞介素6及髓样细胞白血病1表达相关性及临.docVIP

人类宫颈癌中白细胞介素6及髓样细胞白血病1表达相关性及临 　【摘要】 目的 研究 白细胞介素6(IL6)、髓样细胞白血病1(MCL1)蛋白及MCL1mRNA在宫颈癌、癌前病变及正常宫颈组织中的表达情况及其相关性。 方法 采用免疫组化法检测30例宫颈鳞癌、10例宫颈上皮内瘤变(CIN)及10例正常宫颈组织中IL6和MCL1蛋白表达情况，采用逆转录PCR法检测组织中MCL1 mRNA的表达情况。结果 IL6和MCL1蛋白在宫颈癌组织中的表达高于正常宫颈组织及CIN组织(Plt;0.01)，且IL6和MCL1蛋白的表达呈正相关性(r=0.566，Plt;0.01)；MCL1蛋白与MCL1mRNA表达呈正相关性(r=0.772，Plt;0.01)。结论 IL6和MCL1的表达在宫颈癌的发生、 发展 过程中起着共同调节作用，这一发现有助于对早期癌前病变的筛查，并为宫颈癌的 治疗 提供了一个细胞因子靶点。 【关键词】 白细胞介素6；髓样细胞白血病1；宫颈癌；凋亡 　　 The correlation and clinical significance of interleukin6 and　myeloid cell leukelia1 expression in human cervical cancer 　　　ABSTRACT: Objective To investigate the expression and correlation of interleukin6 (IL6), myeloid cell leukelia1 (MCL1) protEin and MCL1mRNA in cervical cancer, cervical intraepithelial neoplasia (CIN) and normal cervix. Methods Immunohistochemistry was used to detect the expression of IL6 and MCL1 protEIn in 30 samples of cervical squamous cell carcinoma, 10 samples of CIN and 10 samples of normal cervix, and RTPCR was used to detect the expression of MCL1mRNA. Results The protein levels of IL6 and MCL1 were significantly higher in cervical cancer tissues than in normal cervix and CIN tissues (Plt;0.01), and MCL1 protein expression was positively correlated to IL6 protein expression (r=0.566, Plt;0.01); MCL1mRNA expression was positively correlated to MCL1 protein expression (r=0.772, Plt;0.01). Conclusion IL6 and MCL1 expression probably play an important regulating role in the pathogenesis and development of cervical cancer. It is conducive to screening the precancerosis and providing a cell factor target for the treatment of cervical cancer. 　　KEY WORDS: interleukin6; myeloid cell leukelia1; cervical cancer; apoptosis 毕业论文 　　白细胞介素6(interleukin6)是具有多种生物活性的多能细胞因子，同时也是女性生殖道感染中重要的促炎症因子，其对宫颈癌细胞的增殖和血管生成有重要 影响 [1]。体外研究表明，IL6可能还存在一定的抗凋亡作用，即通过改变BCL2家族蛋白的水平来调节凋亡[2]。髓样细胞白血病1(myeloid cell leukelia1)在人类宫颈癌组织中的表达是BCL2家族抗凋亡蛋白中最丰富的[3]。 目前 国内关于宫颈癌细胞中IL6与MCL1的相关性尚未