六味地黄汤对雌性致衰模型小鼠生殖器官影响实验探究.docVIP

六味地黄汤对雌性致衰模型小鼠生殖器官影响实验探究 　【摘要】 目的观察六味地黄汤（LW）对雌性致衰小鼠生殖器官的影响。方法小鼠颈背部皮下注射D - 半乳糖建立衰老模型，同时给予六味地黄汤、己烯雌酚治疗，然后检测六味地黄汤对β-内啡肽（β-EP）、血清雌激素（E2）、孕激素（P）含量以及对卵巢、子宫形态学的影响。结果六味地黄汤能够提高β-内啡肽含量，增加小鼠阴道脱落细胞涂片内角化上皮细胞和表层细胞，卵巢重量增加。结论六味地黄汤具有雌激素样作用，可能通过调理下丘脑-垂体-卵巢轴(HPO)的功能，使卵巢功能恢复。 【关键词】 六味地黄汤； 卵巢早衰 　　Abstract：ObjectiveTo observe the effects of Liuwei Dihuang Tang on causing senility to ovaria of mice. MethodsTo establish senility model through subcutaneous injection of D-galactogen into the neck and back of mice, which were subsequently treated with Liuwei Dihuang Tang and ethylene cyclohexne. Meanwhile, to observe t