加味六味地黄汤对环磷酰胺致幼年大鼠肝损害保护作用.docVIP

加味六味地黄汤对环磷酰胺致幼年大鼠肝损害保护作用 　 作者：姜荣燕， 朱立平， 陈述英， 梁秀玲， 李然然 【摘要】 目的 探讨加味六味地黄汤对环磷酰胺致幼年大鼠肝损害的保护作用。方法 2周龄及3周龄Wistar大鼠各32只，每一周龄段按随机数字表法各分为3组，正常组8只，对照组12只和实验组12只。实验组灌胃加味六味地黄汤，2周龄大鼠咽饲量为每只每次0.6 mL，3周龄咽饲量为每只每次0.9 mL，每日2次，连续14 d。对照组根据大鼠周龄喂饲等量生理盐水，每日2次，连续14 d。实验组和对照组第7天和第8天腹腔注射环磷酰胺100 mg/(kg·d);正常组常规喂养，第7天和第8天腹腔注射等量生理盐水。3组均于第15天检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的水平。苏木精-伊红染色镜检观察肝组织病理学改变。结果 2周龄及3周龄对照组大鼠ALT、AST均明显高于正常组，差异有统计学意义(Plt;0.05)，且实验组ALT、AST均明显低于对照组，差异有统计学意义(Plt;0.05);光镜下对照组肝细胞广泛性的中度至重度水变性，实验组肝细胞水肿明显减轻。结论 加味六味地黄汤对环磷酰胺致幼年大鼠肝损伤有一定的保护作用。 【关键词】 加味六味地黄汤/治疗应用; 环磷酰胺/中毒; 肝/损伤; 大鼠，Wistar; 动物，实验 　　【Abstract】 Objective To explore the protective effect of Jiawei Liuwei Dihuang recipe on the hepatic injury of the young rats induced by cyclophosphamide(CP).Methods A total of 32 2weekold and 3weekold Wistar rats were divided into three groups, each weekold segment according to the random number table, normal group 8, control group 12 and experimental group 12. Experimental group was fed Jiawei Liuwei Dihuang recipe, 2weekold rats were fed pharyngeal volume 0.6 mL for each, for 3 weeks of age,0.9 mL，twice a day for 14 days. The control group rats were fed normal saline according to weeks, twice a day for 14 days. The experimental group and control group were intraperitoneally injected cyclophosphamide 100 mg/(kg·d) on the 7th day and 8th day; with normal routine feeding, equivalent normal saline was intraperitoneally injected on the 7th day and 8th day for the normal group. Serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were detected on the 15th day. After hematoxylineosin staining, the pathological changes in liver tissue were observed by microscope.Results The ALT and AST of the 2weekold and 3weekold control rats were significantly higher than the normal group, the difference being of statistical significance(Plt;0.05);ALT and AST of the experimental group were significantly lower than the control group, the difference being of statistical significance(Plt;0.0