卵裂球存活状况对冻融胚胎种植率影响.doc

卵裂球存活状况对冻融胚胎种植率影响 　 作者：罗亚宁，王晓红，李丽，杨艳红，黄剑磊，张远强，姚元庆 【关键词】 冻融胚胎；卵裂球存活；胚胎移植；种植率 　　Impact of blastomere survival on implantation rate of frozenthawed embryo transfer 　　【Abstract】 AIM: To study the impact of blastomere survival on the implantation rate of frozenthawed embryo transfer (FET). METHODS：We conducted a retrospective analysis of 134 FET cycles from September 2003 to September 2005. According to blastomere survival rates, all FET cycles were categorized into 3 groups: group A (n=46) included cycles in which all their original blastomeres remained intact；group B (n=65) refered to mixed transfer of fully intact embryos together with partially damaged ones；group C (n=23) was composed of all transferred embryos with partially damaged blastomere. RESULTS: Out of 642 thawed embryos, 455 survived, survival rate being 70.8%. The pregnancy rate was 24.6% per FET cycle. The pregnancy rate and the implantation rate of group A, B,and C were respectively 34.7% and 18.5%, 23.1% and 8.2%, 8.7% and 3.8%. There was a significantly higher implantation rate in group A than in group C（Plt;0.05）. Along with the increase of intact embryos in FET cycles (0, 1, 2, 3), there was a significant increase in the pregnancy rate （8.7%， 15.0%， 31.4%， 38.9%） and the implantation rate（3.8%， 6.1%， 16.2%， 18.5%, Plt;0.05）. CONCLUSION：Blastomere survival status is a most important factor influencing the implantation rate of frozenthawed embryo transfer. 　　【Keywords】 frozenthawed embryo；survival blastomere；embryo transfer；implantation rate 　　【摘要】 目的：观察冻融胚胎卵裂球存活状况与冻融胚胎种植率的关系. 方法：对134个冻融胚胎移植周期进行回顾性分析. 根据冻融胚胎移植周期中卵裂球存活状况分为3组：A组(n=46)为所有移植胚胎卵裂球全部存活；B组(n=65)为混合移植；C组(n=23)为所有移植胚胎均有卵裂球受损. 结果：在134个FET治疗周期中，共解冻胚胎642个，复苏胚胎455个. 有33个周期获临床妊娠，每个冻融胚胎移植周期的临床妊娠率为24.6%. A组的临床妊娠率和种植率为34.7%和18.5%，B组的临床妊娠率和种植率为23.1%和8.2%，C组的临床妊娠率和种植率为8.7%和3.8%. A组种植率明显高于C组（Plt;0.05）. 随着移植胚胎中含100%卵裂球存活胚胎数的增加（0，1，2，3），其临床妊娠率（8.7%，15.0%，31.4%，38.9%）和种植率（3.8%，6.1%，16.2%，18.5%）均明显增加（Plt;0.05）. 结论：卵裂球的存活状况是影响冻融胚胎种植率的重要因素. 　　【关键词】 冻融胚胎；卵裂球存活；胚