2-aminoacetophenone as a potential breath biomarker for pseudomonas aeruginosa in the cystic fibrosis lung2-aminoacetophenone呼吸作为一个潜在的生物标志物的铜绿假单胞菌肺囊性纤维化.pdfVIP

Scott-Thomas et al. BMC Pulmonary Medicine 2010, 10:56 /1471-2466/10/56 RESEARCH ARTICLE Open Access 2-Aminoacetophenone as a potential breath biomarker for Pseudomonas aeruginosa in the cystic fibrosis lung 1* 2 1 3 3 1 Amy J Scott-Thomas , Mona Syhre , Philip K Pattemore , Michael Epton , Richard Laing , John Pearson , Stephen T Chambers3 Abstract Background: Pseudomonas aeruginosa infections are associated with progressive life threatening decline of lung function in cystic fibrosis sufferers. Growth of Ps. aeruginosa releases a “grape-like” odour that has been identified as the microbial volatile organic compound 2-aminoacetophenone (2-AA). Methods: We investigated 2-AA for its specificity to Ps. aeruginosa and its suitability as a potential breath biomarker of colonisation or infection by Solid Phase Micro Extraction and Gas Chromatography-Mass Spectrometry (GC/MS). Results: Cultures of 20 clinical strains of Ps. aeruginosa but not other respiratory pathogens had high concentrations of 2-AA in the head space of in vitro cultures when analysed by GC/MS. 2-AA was stable for 6 hours in deactivated glass sampling bulbs but was not stable in Tedlar® bags. Optimisation of GC/MS allowed detection levels of 2-AA to low pico mol/mol range in breath. The 2-AA was detected in a significantly higher proportion of subjects colonised with Ps. aeruginosa 15/16 (93.7%) than both the healthy controls 5/17 (29%) (p 0.0002) and CF patients not colonised with Ps. aeruginosa 4/13(30.7%) (p 0.001). The sensitivity and specificity of the 2-AA breath test compared to isolation of Ps. aeruginosa in sputum and/or BALF was 93.8% (95% CI, 67-99) and 69.2% (95% CI, 38-89) respectively. The peak integration value