右旋柠烯对HL-60、K562白血病细胞体外作用探究.docVIP

右旋柠烯对HL-60、K562白血病细胞体外作用探究 　 ; 作者：郭晓铭，路晴，刘正娟，王莉芬，冯秉安 毕业论文 【摘要】; 　　本研究观察右旋柠烯(d-limonene，D-L)对HL-60和K562白血病细胞增殖凋亡的影响，并进一步探讨其作用机制。首先采用碘化丙锭染色法观察右旋柠烯对HL-60、K562细胞增殖的影响，再通过细胞形态学观察、流式细胞术分析、免疫细胞化学半定量测定突变型p53、bcl-2、bax基因的表达水平，系统观察D-L对HL-60、K562细胞体外诱导凋亡的情况。结果表明： D-L抑制HL-60和K562细胞的增殖，IC50均为0.75 mmol/L，呈剂量时间依赖关系； D-L诱导HL-60、K562细胞凋亡；D-L在诱导HL-60细胞凋亡过程中随作用浓度增加，bcl-2的表达下降；D-L在诱导K562细胞凋亡过程中随作用浓度增加，突变型p53及bcl-2的表达下调，而bax的表达上调。结论： D-L抑制HL-60和K562细胞增值并诱导其凋亡；突变型p53，bcl-2，bax参与了D-L诱导凋亡的基因调控。 毕业论文 【关键词】; 右旋柠烯； HL-60； K562细胞； 细胞增值； 细胞凋亡 毕业论文 　　Effects of D-limonene on Leukemia Cells HL-60 and K562 in Vitro 毕业论文 　　Abstract To investigate the effects of D-limonene(D-L) on the cell growth and apoptosis in HL-60，K562 cells and to elucidate its mechanism，the influence of D-L on proliferation of HL-60 and K562 cells was determined by propidium iodide assay，the expression levels of mutant p53，bcl-2，bax gene were detected by cell morphological analysis，flow cytometry and immunohistochemistry staining，the D-L-inducing HL-60 and K562 cell apoptosis in vitro was observed systematically. ; The results showed that D-L inhibited HL-60 and K562 cell growth in a dose-and time-dependent manner with the IC50 of 0.75 mmol/L similarly，D-L induced apoptosis of HL-60 and K562 cells，and ; expression of; bcl-2 gene was down regulated by D-L in a concentration-dependent manner in HL-60 cells.The bcl-2，mutant type of p53 genes were down regulated while bax gene was up regulated by D-L in a concentration-dependent manner in K562 cells.; It is concluded that D-L can; inhibit proliferation and induce apoptosis of HL-60 and K562 cells. The bcl-2，mutant type of p53 and bax may be involved in the gene regulation of D-L-induced apoptosis. 毕业论文 　　Key words D-limonene; HL-60，K562; Cell proferation; Apoptosis 毕业论文 　　右旋柠烯（D－limonene，D-L）是一种天然单萜烯类化合物，在桔皮精油中的含量gt;90%，学名1-甲基-4-异丙基环己烯，分子式C10H16，分子量136.23。D-L是limonene中最普遍存在的一种异构体，具有新鲜柠檬香气和抗氧化、抗炎、利胆溶石等作用。近年发现D-L具有抗癌活性，用于预防及治疗化学致癌物诱导的结肠癌、乳腺癌、胃癌、肺癌、皮肤癌、肝癌、胰腺癌等取得了相当的效果