原创力文档a boundary delimitation algorithm to approximate cell soma volumes of bipolar cells from topographical data obtained by scanning probe microscopy划定的边界算法近似细胞soma的双极细胞从地形数据获得的扫描探针显微镜.pdfVIP
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a boundary delimitation algorithm to approximate cell soma volumes of bipolar cells from topographical data obtained by scanning probe microscopy划定的边界算法近似细胞soma的双极细胞从地形数据获得的扫描探针显微镜
Happel et al. BMC Bioinformatics 2010, 11:323
/1471-2105/11/323
M E T H O D O L O G Y A R T I C L E Open Access
Methodology article
A boundary delimitation algorithm to approximate
cell soma volumes of bipolar cells from
topographical data obtained by scanning probe
microscopy
1,2 2,3 1 2
Patrick Happel* , Kerstin Möller , Ralf Kunz and Irmgard D Dietzel
Abstract
Background: Cell volume determination plays a pivotal role in the investigation of the biophysical mechanisms
underlying various cellular processes. Whereas light microscopy in principle enables one to obtain three dimensional
data, the reconstruction of cell volume from z-stacks is a time consuming procedure. Thus, three dimensional
topographic representations of cells are easier to obtain by scanning probe microscopical measurements.
Results: We present a method of separating the cell soma volume of bipolar cells in adherent cell cultures from the
contributions of the cell processes from data obtained by scanning ion conductance microscopy. Soma volume
changes between successive scans obtained from the same cell can then be computed even if the cell is changing its
position within the observed area. We demonstrate that the estimation of the cell volume on the basis of the width
and the length of a cell may lead to erroneous determination of cell volume changes.
Conclusions: We provide a new algorithm to repeatedly determine single cell soma volume and thus to quantify cell
volume changes during cell movements occuring over a time range of hours.
Background A promising approach to circumvent these problems is
Cell volume regulation occurs in a wide variety of tissues to measure volume directly with a scanning
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