大鼠体力及精神状态评价行为学新技术.docVIP

大鼠体力及精神状态评价行为学新技术 　【摘要】 目的：改良传统鼠尾悬吊试验（Tail Suspension Test，TST），通过计算机精确地测定及评估大鼠体力及精神状况，实现行为学的计算机辅助化。方法 ：以大中型手术创伤复制体力及精神低下的动物模型，41只大鼠随机分为手术创伤组及对照组，并以这两组为验证对象。以Medlab-U多导生物信号记录仪结合传统鼠尾悬吊试验，以计算机辅助化的电子信号波形式记录处于倒挂厌恶条件下大鼠因反抗不适体位或试图逃脱而挣扎时释放的动能及势能，并依据释放的能量提出挣扎域上面积、累积不动时间和单次最长静止时间等三大指标评价该两组大鼠体力耐力及精神方面的差异。结果 ：改良法三大指标验证手术创伤组术后改变，在该实验中以上三大指标都有显著性差异（P lt;0.05或P lt;0.01）；与传统法相比，改良法不仅能排除实验人员主观因素导致的误差，使实验结果客观化，增加可信度，而且提高了动物行为学测定效率，更实现了动物行为学试验的自动化、量化。结论 ：改良法用于测定及评估各种大鼠模型体力及精神情况，方法简单，结果可靠。 【关键词】 鼠尾悬吊试验；体力；精神；行为学；大鼠 Abstract: Objective: To evaluate and determine the physical strength and vim state of mice accurately in a computer-assisted way by improving the traditional tail suspension test (improved TST)，make the idea of computer-assisted etholgical test come ture. Methods:Building the model of low level physical strength and vim state by severe surgical trauma，dividing the 41 mice into surgical trauma group and control group as experimental subjects.Combining the multiplying channel biological signal recorder-Medlab U with the traditional TST to record the signal waves coming from the kinetic and potential energy which produced by the struggling of the suspending mice which trying to escape under aversion condition. Evaluate the mice by Area Above Threshold of Struggle；Accumulated Immobile Time；Longest Single Static Time. Results:The improved way could validate the postopretive change well in ST group，comparing with traditional TST，the 3 indicators all come with a significant difference in the research（P lt;0.05or P lt;0.01）. This improvement could remove subjective factors of the handlers. Not only makes the test effective，but also automated，objective，quantificat. Conclusion:This improvment can determine and evaluate animal model physical strength and vim state，it has easy method and confident result，and the generalization is deserving. Key words: tail suspension test；vim state；ethology；mice 在动物医学科研活动中，研究者常需从整体水平评估动物体力、耐力及精神状况，而此类行为学测试方法不多，其中以传统鼠尾悬吊试验（Tail Suspens