大肠埃希菌产PER-1型超广谱β-内酰胺酶探究.docVIP

大肠埃希菌产PER?1型超广谱β?内酰胺酶探究 　 【摘要】; 目的 检测大肠埃希菌产PER?1型超广谱β?内酰胺酶的发生率及其耐药特点。方法 采用双纸片增效确证试验进行超广谱β?内酰胺酶(ESBLs)的表型检测，对表型阳性菌株用聚合酶链反应(PCR)和PCR产物测序方法确定超广谱β?内酰胺酶的基因型。结果 167株大肠埃希菌中有50株产ESBLs，占29.9%(50/167)；6株产PER?1型ESBLs，占3.6%(6/167)。6株产PER?1型ESBLs大肠埃希菌均对头孢他啶和头孢噻肟耐药，5株对头孢西丁耐药，4株对阿米卡星敏感，全部菌株对亚胺培南敏感。结论 大肠埃希菌中检测到产PER?1型超广谱β?内酰胺酶，且其耐药和多重耐药性严重，应引起重视。 毕业论文 【关键词】; 大肠埃希菌 PER?1 β?内酰胺酶 耐药基因型 毕业论文 ; Study on PER?1 type extended?spectrum β?lactamase produced 毕业论文 ; ABSTRACT; Objective; To investigate the prevalence of PER?1 type extended spectrum β?lactamases (ESBLs) and its antimicrobial?resistance profile in Escherichia coli.; Methods; One hundred and sixty?seven strains of Escherichia coli were tested. All of them were isolated from all kinds of clinical specimens from four hospitals of Nanchang city from August 2002 to June 2003. Antimicrobial susceptibility tests (AST) were determined by Kirby?Bauer disk diffusion test. Double?disk test and three?dimension extraction test were used to determine ESBLs. blaPER?1 genes were amplified by PCR. The products of PCR were sequenced to identify the β?lactamase genotype.; Results; The positive rates of ESBLs in Escherichia coli were 29.9% (50/167). Six isolates carried blaPER?1 gene. The blaPER?1 gene positive rates of Escherichia coli is 3.6% (6/167). Among 6 isolates carring blaPER?1, all were resistant to cefotaxime and ceftaxidime, 2 resistant to amikacin, 5 resistant to cefoxitin and all sensitive to imipenem.; Conclusion; PER?1 β?lactamases and their encaling genes are detected in Escherichia coli. The isolates harbouring blaPER?1 are resistant to many antibiotics. 毕业论文 ; KEY WORDS; Escherichia coli PER?1 β?lactamases Resistance genotype收稿日期：2006?04?18 毕业论文 ; 大肠埃希菌是临床常见病原菌，特别是其高产超广谱β?内酰胺酶(ESBLs)及多重耐药性己引起临床广泛关注，产ESBLs大肠埃希菌株不断增多，且可通过质粒造成耐药性的跨菌种播散，对医院感染构成严重威胁。1993年Nordmann等首次从法国分离的铜绿假单胞菌中检出PER?1型ESBLs，1997年Vahaboglu等［1］在土耳其的一次普查中发现72株不动杆菌中有33株产PER?1型ESBLs， 检出率达45.8%，2002年我国蒋晓飞等［2］在鲍曼不动杆菌中检出PER?1型ESBLs。目前，关于肠杆菌科细菌产PER?1型ESBLs研究