大鼠窦房结周围组织缝隙连接蛋白Cx43表达增龄性变化.docVIP

大鼠窦房结周围组织缝隙连接蛋白Cx43表达增龄性变化 　 作者：程功，孙超峰，黄欣，杜媛，张军波，王永红 【关键词】 窦房结；年龄因素；,Cx43；窦房结传导时间 　　Agerelated changes of Cx43 proteins in the region around the sinoatrial node of rats 　　【Abstract】 AIM: To observe the change of sinoatrial conduction time(SACT) and protein expression level of Cx43 in the region around sinoatrial nodes(SAN) in neonatal, adult and old SD rats, and to explore the molecular basis of SAN electrophysiological reconstruction. METHODS: The rats were anesthetized, and then ECG was recorded. Esophageal electrode was inserted to count SACT. The SAN was separated and prepared into 10 μm continuous frozen sections. Four neighboring sections were taken as a group, and then HE and Masson stainings were performed at the 2 middle sections. Immunohistochemical technology was used for the other 2 sections by FITC marked antibody of HCN4 and Cx43. Cx43 was measured with laser confocal microscope. RESULTS: ① SACT(ms) was prolonged with ageing（neonatal 15.2±2.2, adult 17.6±1.6, old 19.3±0.9）. ② The expression of Cx43 in the region around SAN decreased with ageing（CT: neonatal 21.3±3.9, adult 15.7±2.7, old 6.9±1.8; Sinus: neonatal 14.2±4.2, adult 10.7±3.0, old 5.3±2.1）. CONCLUSION: Cx43 in the region around SAN possibly participates in electrophysiological molecular mechanism of agerelated SAN functional regression. 　　【Keywords】 sinoatrial node (SAN); age factors; Cx43; SACT 　　【摘要】目的： 研究幼年、成年和老年SD大鼠窦房结传导时间（SACT）变化，检测窦房结周围组织缝隙连接蛋白Cx43的表达，部分探讨窦房结电生理重构的分子基础. 方法： 大鼠麻醉后行心电图检查，放置食道电极，测量SACT. 分离窦房结及其周围组织，制备成10 μm厚的连续冰冻切片. 相邻4张为一组，分别行HE染色、Masson染色、FITC标记抗Cx43和抗HCN4的免疫组化法染色，激光共聚焦显微镜测量Cx43荧光强度. 结果： ① 随年龄增长，SACT(ms) 延长（幼年15.2±2.2, 成年17.6±1.6, 老年19.3±0.9）. ② 窦房结周围组织Cx43表达随年龄增长逐渐降低，（界嵴处： 幼年组21.3±3.9，成年组15.7±2.7，老年组6.9±1.8；腔静脉窦：幼年组14.2±4.2，成年组10.7±3.0，老年组5.3±2.1）. 结论： 窦房结周围组织Cx43表达增龄性降低可能是构成窦房结功能增龄减退的一个重要的电生理分子基础. 　　【关键词】 窦房结；年龄因素； Cx43；窦房结传导时间 　　0引言 　　病态窦房结综合征（SSS）是由多种原因引起的窦房结起搏及冲动传导功能障碍而导致的临床综合征. 冲动传导功能障碍主要表现为窦房结的冲动在窦房结内传导延缓或阻滞和冲动传导到组织结构不完全相同的周围心房组织过程中发生延缓或阻滞，缝隙连结的主要功能是介导细胞之间电和化学信号的传