川芎嗪对大鼠肠缺血再灌注肝损伤保护作用实验探究.docVIP

川芎嗪对大鼠肠缺血再灌注肝损伤保护作用实验探究 　 作者：高卉， 阮明凤， 白育庭， 万敬之 【关键词】 川芎嗪；,,肠缺血再灌注损伤；,,小肠；,,肝脏 　　摘要：目的探讨川芎嗪对大鼠肠缺血再灌注肝损伤的保护作用。 方法 选用健康Wistar大鼠24只，随机分成3组（每组8只）：假手术对照组、缺血再灌注组、川芎嗪 治疗 组。夹闭大鼠肠系膜上动脉（SMA）60 min造成缺血，再灌注2 h后取出肝组织制成匀浆，测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化酶（GSHPX）、丙二醛（MDA）、Ca2+Mg2+ATP酶的含量及肝形态细胞学变化。结果川芎嗪治疗组肝组织MDA含量明显低于缺血再灌注组（P＜0.01），SOD，GSHPX，Ca2+Mg2+ATP酶的含量均明显高于缺血再灌注组（P＜0.01），肝细胞形态学异常变化明显减轻。结论川芎嗪能减少大鼠脂质过氧化，改善ATPase功能，减轻大鼠肠缺血再灌注所致肝脏损伤。 　　关键词：川芎嗪； 肠缺血再灌注损伤； 小肠； 肝脏 论文代写 　　Protective Effects of Tetramethylpyrazine on Hepatic Damage Induced by Intestinal Ischemiareperfusion in Rats 　　Abstract:ObjectiveTo explore the protective effects of tetramethylpyrazine on hepatic damage induced by intestinal ischemiareperfusion in rats. MethodsDivide the rats randomly into three groups: ⑴sham operated group, ⑵untreated ischemiareperfution(I/R) group, ⑶pretreatment with tetramethylpyrazine before I/R(Te). Superior mesenteric artery was clipped for 60 min to cause ischimia, and then unclipped to make reperfusion for 2 hours after reperfusion, the liver tissues were gathered and homogenized respectively, and the contents of SOD, GSHPX, MDA and ATPase,and morphological changes of hepatocytes were assayed.Results The contents of MDA in liver in Te groups were less than that in I/R group （P＜0.01）,the contents of SOD,GSHPX and Ca2+,Mg2+,ATPase in Te groups were higher than those in I/R group（P＜0.01）,and the abnormal morphological changes of hepatocytes diminished remarkably. ConclusionTetramethylpyrazine can reduce lipid peroxidation, improve ATPase activity and decrease the injury of liver tissue in the rats after intestinal ischemiareperfusion. 　　Key words:Tetramethylpyrazine; Ischemiareperfusion injury; Gut; Liver 　　肠缺血再灌注（ischemiareperfusion，I/R）是机体遭受严重创伤、大手术、烧伤、感染等刺激后发生较早的病理过程之一，可引起肝、肾、肺等远隔器官的损伤［1］。 目前 认为，缺血再灌注损伤产生的机理主要与氧自由基大量生成导致组织器官脂质过氧化有关［2］。川芎嗪（Tetramethylpyrazine，TMP）是从川芎中提出的一种生物碱，具有解痉、扩管、降压等广泛的生物作用，主要 应用 于心血管疾病的治疗［3］。本 研究 旨在探讨川芎嗪对大鼠肠缺血再灌注肝损伤的保护作用，并探讨其可能机制。 　　1 材料与方法 论文代写 　　1.1 药品及试剂