急性白血病患者氧化应激状态及抑郁情绪之间关系.doc

急性白血病患者氧化应激状态及抑郁情绪之间关系 　 作者：周芙玲，张王刚，苏智祥，蒙昕，田纬 毕业论文 【摘要】; 本研究探讨急性白血病患者氧化应激状态与抑郁情绪之间的关系。用抑郁自评量表（self-rating depression scale， SDS）等调查表对92例初发急性白血病患者的抑郁症状进行评定。检测血浆总抗氧化能力（T-AOC）、活性氧(ROS)、超氧化物歧化酶（SOD）、丙二醛(MDA)及一氧化氮（NO）含量。采用实时定量PCR检测DNA修复基因人8- 羟基鸟嘌呤糖苷酶（human 8-hydroxyguanine glycosylase，hOGG1）的表达。结果证明，急性白血病患者抗氧化酶防御能力下降，抑郁情绪发生率47.83%，伴抑郁障碍的患者T-AOC和SOD水平明显降低，ROS、NO及MDA水平明显升高，DNA修复基因hOGG1 mRNA的表达升高。急性白血病患者病程、hOGG1水平与抑郁程度呈正相关，T-AOC水平与抑郁程度呈负相关。结论： 急性白血病患者存在氧化损伤，伴抑郁症状的患者抗氧化酶防御能力进一步减弱,提示氧化应激与急性白血病抑郁症状的发生和发展密切相关。 毕业论文 【关键词】; 氧化应激；急性白血病；抑郁；DNA修复基因hOGG1 毕业论文 　　Relationship between Oxidative Stress and Depression in Patients with Acute Leukemia 毕业论文 　　AbstractThis; study was aimed; to investigate the relationships between oxidative stress and depression in patients with acute leukemia. Ninety two cases of; acute leukemia; were randomly enrolled in the study. Depressive disorder was assessed by self-rating depression scales (SDS) and multiple items questionaires.; The; total anti-oxidation capability (T-AOC), reactive oxygen species (ROS); and; superoxide dismutase (SOD); activities, as well as malondialdehyde (MDA) and nitric oxide (NO) levels were measured in pre-treatment periods. Meanwhile, the steady state level of human 8-hydroxyguanine glycosylase（hOGG1）mRNA transcript was monitored by quantitative real-time PCR. The results showed that the defence of antioxidant system was impaired in patients with; acute leukemia. The incidence of depression was 47.83% in 92 cases. T-AOC and SOD activities were significantly decreased in patients with depression，while ROS, NO, MDA levels and hOGG1 mRNA expression were reverse of the former. It revealed that depression; positively correlated with course of disease and hOGG1, and negatively correlated with T-AOC. It is concluded that oxidative damage occurs in patients with; acute leukemia, moreover, lower antioxidant defences exist in depressive patients. These results underscore the notion that oxidative stress may promote the d