拘束冷应激对大鼠肾脏热休克蛋白表达及血清皮质醇含量动态影响.doc

拘束冷应激对大鼠肾脏热休克蛋白表达及血清皮质醇含量动态影响 　作者：吴步猛，林飞燕，赵惠玲，黄卡特，林婷婷，包晶莹，吴军，方舟溪，陈国荣 【摘要】 目的 ：研究拘束冷应激对大鼠肾脏组织热休克蛋白(heat shock protein 70，HSP70)表达和血清中皮质醇(Cortisol，CORT)含量的动态影响及可能的机制。方法：选取50日龄清洁级大鼠77只，随机分为7组，即0、0.5、1.0、1.5、2.0、2.5和3.0 h。采用拘束冷应激法，应激相应时间后，用免疫组织化学方法观察大鼠肾脏组织HSP70的表达，并检测血清中CORT含量。结果：血清中的CORT浓度在1.5 h呈现最高值，其余各时点变化不明显。光镜下可见HSP70在肾小球毛细血管内皮细胞的胞核及肾小管上皮细胞的胞核和胞浆中均有表达。肾脏中HSP70的表达首先呈增加的趋势，1.5～2.5 h HSP70持续高表达(Plt;0.01)，之后下降(Plt;0.05)。结论：适当强度的拘束冷应激能诱导肾脏组织中HSP70的合成，并且随着时间的延长而增加，但过度的拘束冷应激则可能降低或阻断肾脏HSP70的表达。 【关键词】 拘束冷应激;大鼠;肾脏;热休克蛋白;皮质醇 　Abstract: Objective: To study the dynamic effects of cold restraint stress on HSP70 expression　in kidney and serum corticosteroid content in rats， and explore its possible mechanism.　Methods: Seventy-seven 50-day-old SD rats were randomly divided into 7 groups，namely 0 h，0.5h，1.0 h，1.5 h，2.0 h，2.5 h and 3.0 h group. At corresponding restraint stress time，the expressionof HSP70 in kidney tissues were tested by means of immunohistochemistry and the level of CORTin serum was also examined. Results: The level of CORT reached its peak in 1.5 h group， but therewas no significant difference in other groups. Under the light microscope， the expressions ofHSP70 in the nuclei of renal glomerular capillary endothelial cells，in the nuclei and cytoplasmof renal tubular epithelial cells could be observed. The HSP70 expression in kidney increasedcontinuously first，then it was kept in a high level at stress time of 1.5 h， 2.0 h and 2.5 h(Plt;0.01)，and since then it declined gradually(Plt;0.05). Conclusion: Suitable cold restraint　stress can induce the synthetic of HSP70， and it increased with stress time. While excessive　　cold restraint stress may decreased or block the expressions of HSP70 in kidney. 　　Key words: cold restraint stress;rats;kidney;heat shock protein 70;Cortisol 　　当动物在各种极端环境因素如雪灾、地震等造成的寒冷束缚的刺激下，机体的组织、器　　官、细胞内会产生各种类型的应激变化，这些变化能够反映动物的生理状态和健康状态。近　　年来的研究发现，在热暴露、冷暴露和损伤等应激条件下，一组应激蛋白热休克蛋白家族　　(heat shock proteins，HSPs)的表达水平会显著增加，HSPs在机体内作为一种分子伴侣