摄入石榴汁时间长短对衰老小鼠 抗氧化功能影响.doc

摄入石榴汁时间长短对衰老小鼠 抗氧化功能影响 　 【摘要】; 探讨石榴果汁在衰老小鼠体内的抗氧化功能。方法 取72只老龄小鼠，雌雄各半，将动物按体重随机分为高剂量组、低剂量组、对照组，剂量组每日分别按照20?mL/kg石榴汁、10?mL/kg石榴汁进行灌胃，对照组按20?mL/kg蒸馏水进行灌胃。分别于第1、2、3、4周结束时每组取6只小鼠拔眼球取血并脱臼处死，3?500?r/min离心15?min后分离血清，立即装于eppendorf管中，置-20?℃保存待用测定总抗氧化能力（T?AOC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶(GSH?PX)、丙二醛（MDA）；并取肝脏快速冷冻用于T?AOC、SOD、GSH?PX、MDA的测定。结果 与对照组比较，前两周高剂量组小鼠与对照组小鼠的总抗氧化能力的差异无统计学意义，第3周两者的差异开始有统计学意义。高剂量组和低剂量组小鼠血清和肝脏总抗氧化能力、SOD和GSH?PX活力显著提高；血清和肝脏MDA含量降低不明显。高剂量组作用较低剂量组显著。结论 石榴汁具有良好的抗氧化功能，随着石榴汁摄入时间的延长，衰老小鼠机体的抗氧化水平逐渐增强。 毕业论文 【关键词】; 石榴 总抗氧化能力 超氧化物歧化酶 谷胱甘肽过氧化物酶 丙二醛 毕业论文 ; different times on aging mice 毕业论文 ; Abstract: Objective; To explore the anti?oxidation effect of pomegranate juice on aging mice. Methods; 72 aging mice, half female and half male, were randomly divided into three groups: the high dose group, the low dose group and the control group. Pomegranate juice or water was given orally every day at the following doses of 20?ml/kg pomegranate juice, 10?ml/kg pomegranate juice and 20?ml/kg distilled water.; 6 mice from each group were taken, their eyeballs were removed, and they were executed with luxation at the end of the first week, the second week, the third week and the fourth week. Blood serum was separated after 3?500?r/min centrifugalization for 15 minutes, and was conserved under refrigeration for determining the total anti?oxidation capability (T?AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH?PX) and malonaldehyde (MDA). Also the liver was obtained and frozen for determining the T?AOC, SOD, GSH?Px and MDA. Results; Compared with the control group, the disparity of T?AOC between the high dose group and the control group was not significantly different at the first two weeks, and it began to have significant differences from the third week. T?AOC, SOD and GSH?PX of blood serum and liver in the high dose and the low dose groups remarkably increased. The content of MDA of blood serum and liver degraded with no significant effect. There was a more noticeab