柔肝散结法对肝癌大鼠干预作用探究.docVIP

柔肝散结法对肝癌大鼠干预作用探究 　【摘要】 　　目的 探讨血管内皮生长因子(VEGF) 及环氧合酶-2（COX-2）在肝癌大鼠中的表达以及柔肝散结法对其表达的影响。方法40只雄性SD大鼠，随机分为空白组、模型组、青消方组、青消方防治组（简称“防治组”），每组10只。除空白组外，其余3组大鼠均以含0.05%2-AAF的饲料喂养12周，防治组在诱癌的同时灌服青消方。青消方组于诱癌第4周开始给药，剂量均为25 g·kg-1 ·d-1。12周末观察瘤体积及瘤重量的变化，并采用免疫组织化学方法检测各组大鼠标本中VEGF及COX-2的表达。结果模型组VEGF及COX-2的表达明显高于空白组(Plt;0.01)；青消方组、防治组VEGF及COX-2的表达明显低于模型组(Plt;0.01)；防治组优于青消方组。结论VEGF及COX-2在肝癌大鼠中表达增强，柔肝散结中药青消方能降低肝癌大鼠VEGF及 COX-2的表达，早期治疗效果更佳。 【关键词】 柔肝散结法； 肝癌大鼠； 血管内皮生长因子； 环氧合 　　Abstract：ObjectiveTo approach the expression of the VEGF and COX-2 in rats with liver cancer and the effect of RouGanSanJie Fa(RGSJF) on it.Methods40 SD rats were divided into four groups at random:blank group, model group, QingXiao Fang group, FangZhi group, 10 rats in every group. Except for blank group, the other three groups were fed with bait vessel with 0.05% 2-AAF for 12 weeks. FanZhi group was lavaged with QingXiao Fang when cancer was induced. QingXiao Fang group 25 g·kg·d-1 was treated at the 4th week after cancer was induced. The volume and the weight of neoplasm were observed at the 12th week, and the expression of VEGF and COX-2 in every group was detected by immunehistochemical method.ResultsThe expression of VEGF and COX-2 in model group was obviously higher than that of blank group (P＜0.01). The expression of VEGF and COX-2 in QingXiao Fang group and FangZhi group was obviously lower than that of model group(P＜0.01), FangZhi group surpassed QingXiao Fang group.ConclusionThe expression of VEGF and COX-2 was augmented in rats with liver cancer, QingXiao Fang can depress the expression of VEGF and COX-2 in rats with liver cancer, especially at earlier period. 　　Key words：RouGanSanJie Fa; Rats with liver cancer; VEGF; COX-2 原发性肝癌是我国常见的恶性肿瘤，发病率和死亡率占全部恶性肿瘤的第三位，易发生肝内外转移，预后较差。本实验利用肝癌大鼠模型，观察实验12周末瘤体积及瘤重量的变化，并采用免疫组织化学检测方法，检测血管内皮生长因子(VEGF)及环氧合酶-2（COX-2）在肝癌大鼠中的表达，探讨柔肝散结中药青消方对以上指标的干预作用，明确该方的部分作用途径及可能机制。 　　1 材料与方法 　　1.1 实验用药 　　青消方（由太子参、白芍、绞股蓝、灵芝、冬凌草、鳖甲、蛇六谷等组成）由河北医科大学中医院门诊药房提供；2-乙酰氨基芴(2-AAF)：Sigma公司。 　　1.2 实验过程及观察指标 　　4