柘木多糖及枸杞多糖对尾吊小鼠免疫功能防护效应-.doc

柘木多糖及枸杞多糖对尾吊小鼠免疫功能防护效应* 　 【摘要】; 目的 研究柘木多糖和枸杞多糖对尾吊小鼠细胞免疫功能的防护作用。方法 分别以柘木多糖、枸杞多糖的高、低剂量经口给予尾吊小鼠，MTS比色法测定脾T淋巴细胞增殖，ELISA法测定培养上清中细胞因子分泌评价免疫功能。结果 尾吊7 d后，小鼠脾T淋巴细胞增殖明显降低，细胞因子IL?2和IFN?γ分泌减少，柘木多糖能提高尾吊小鼠的脾T淋巴细胞增殖及IL?2和IFN?γ分泌。结论 柘木多糖对尾吊模拟微重力小鼠细胞免疫功能有一定的防护作用。 毕业论文 【关键词】; 失重模拟 尾吊 柘木多糖 枸杞多糖(LBP 免疫功能 毕业论文 Protective Effects of Cudrania Tricuspidata Polysaccharides and Lycium Barbarium Polysacharides on the Immune Function of Tail?suspended Mice.SONG Jin?ping, ZHANG Hong?yu,QU Li?na, YANG Fen, LIU Shao?fang, WANG Hong?hui,LIU Yue,XIONG Jiang?hui.Space Medicine amp; Medical Engineering,2007,20(6):402~405 毕业论文 ; Abstract:Objective To study the protective effects of Cudrania tricuspidata polysaccharides(CPS) and Lycium barbarium polysacharides(LBP) on the cellular immune function in tail?suspended mice.Methods; Two dosages of; CPS and LBP were administrated to tail?suspended mice orally respectively. Splenic T lymphocyte proliferation were determined by MTS colorimetric assay and cytokines production in supernatants obtained from cultured spleen cells after stimulation with ConA were detected by ELISA assay. Results Splenic T lymphocyte proliferation and the; levels; of IL?2 and IFN?γ decreased significantly in tail?suspended mice 7 d later, which could be enhanced by administration of CPS. Conclusion CPS has protective effects on cellular immunity of tail?suspended mice which is under simulated microgravity. 毕业论文 ; Key words:weightlessness simulation;tail?suspension;cudrania tricuspidata polysaccharides；lycium barbarum polysaccharides;immune function 毕业论文 ; Address reprint requests to:SONG Jin?ping.China Astronaut Research and Training Center.Beijing 100094,China 毕业论文 ; 多糖是由单糖连接而成的多聚物，广泛存在于动物、植物和微生物中，具有较明确的抗肿瘤、提高免疫功能的作用，不同种类的多糖对于细胞免疫功能、体液免疫功能、巨噬细胞功能、NK细胞(natural killer cells)功能分别有不同的激活作用［1?5］。柘木多糖是近年由山东大学徐誉泰教授从柘树根中首先提取并初步研究了其免疫增强功效；枸杞是传统中药，枸杞多糖作为枸杞的有效成份，其免疫调节作用已受到广泛研究和公认［6］。 毕业论文 ; 空间飞行条件下，航天员受到超重、微重力、振动、噪音、辐射、孤独、睡眠缺失等多方面的影响，细胞免疫功能受到抑制［7?9］，航天员容易发生感染性疾病、潜伏病毒活化