桂枝甘草汤方药半仿生提取法药材组合方式优选.docVIP

桂枝甘草汤方药半仿生提取法药材组合方式优选 　【摘要】 目的 优选桂枝甘草汤方药成分提取时药材的最佳组合方式。方法 将方药分成混煎组和桂枝、甘草单煎混合组,用半仿生提取法提取,提取液作甘草次酸、肉桂酸、总黄酮、挥发油、干浸膏的含量测定及50%乙醇精制液的HPLC指纹图谱比较。结果 除干浸膏2种组合提取液含量相近外,其余4个指标成分含量混煎液均明显高于单煎混合液；2种组合提取液的HPLC指纹图谱无明显差异；HPLC梯度洗脱总积分面积及主要特征峰峰面积：混煎液＞单煎混合液。结论 桂枝甘草汤方药成分提取时以桂枝与甘草混煎为佳。 【关键词】 桂枝甘草汤；半仿生提取法；甘草次酸；肉桂酸；黄酮化合物；挥发油；指纹图谱 Abstract：Objective To optimize the best ingredients combination of Guizhi Gancao Decoction. Method The prescription was put into two groups, and extracting by semi-bionic extraction. The contents of Glycyrrhetinic acid, Cinnamic acid, total flavones, volatile oil and dry extract in the each extract were determined, and HPLC fingerprint of the refined by 50% ethanol was compared. Results Except the similar content of dry extract in two kinds of combinations, the other four index components in mixed extract is significantly higher than individual extraction. Two kinds of combinations extract fingerprints of the HPLC had no significant difference. HPLC gradients eluting total score area and main characteristic peak area in mixed extract were higher than individual extraction. Conclusion It is better that Guizhi Gancao Decoction extracted with Guizhi and Gancao mixed. 　 　　Key words：Guizhi Gancao Decoction；semi-bionic extraction；Glycyrrhetinic acid；Cinnamic acid；total flavones；volatile oil；HPLC fingerprint 桂枝甘草汤出自汉代张仲景的《伤寒论》,由桂枝、炙甘草组成,具有补心助阳、生阳化气、补阳扶中的功效。临床用于心率失常具有较好效果。有研究表明,桂枝甘草汤对环磷酰胺诱发的姐妹染色体互换及微核升高具有较强的抑制作用[1-2]。但目前桂枝甘草汤的应用仍以汤剂为主,方药的提取多采用水提法,存在挥发性成分易损失、易霉变、服用不便等缺点。本试验在用均匀设计优选出桂枝甘草汤半仿生提取法(简称SBE法)工艺条件的基础上,将方中药材组合成2组,用优选出的SBE法工艺条件提取,提取液作甘草次酸、肉桂酸、总黄酮、挥发油、干浸膏的含量测定及50%乙醇精制液的HPLC指纹图谱比较,优选出桂枝甘草汤方药提取时药材的最佳组合方式。 　　1 仪器与试药 LC-10A型高效液相色谱仪(日本岛津公司)；754紫外可见分光光度计(上海精密科学仪器有限公司)；PHS-3C型精密PH计(上海雷磁仪器厂)；FA1004型电子分析天平(上海第二分析仪器厂)；LXV-Ⅱ型离心沉淀机(上海第二分析仪器厂)；KQ-250E型医用超声清洗器(昆山市超声仪器有限公司)；色谱柱：phenomenex ODS3(5 μm,4.6 mm×150 mm)；SPD-10Avp紫外检测器；RE-52AA型旋转蒸发器(上海亚荣生化仪器厂)。 　　药材经鉴定,桂枝为樟科植物肉桂Cinnamomum cassia Presl的干燥嫩枝,甘草为豆科植物甘草Glycyrrhiza uralensis Fisch.的干燥根及根茎。炙甘草：取炼蜜,加适量开水稀释后,淋