注射用法罗培南钠对金葡菌体内外抗菌活性探究.docVIP

注射用法罗培南钠对金葡菌体内外抗菌活性探究 　【摘要】 目的 研究注射用法罗培南钠对临床分离的金葡菌的体内外抗菌活性。方法 用头孢西丁纸片法在近年来本室保存的临床分离金葡菌中筛选耐甲氧西林的金葡菌(methicillin resistant staphylococcus aureus，MRSA)，用琼脂平板稀释法测定法罗培南和万古霉素对甲氧西林敏感的金葡菌(methicillin sensitive staphylococcus aureus，MSSA)和MRSA菌株的最低抑菌浓度(minimal inhibitory concentration，MIC)。根据MIC选择对法罗培南敏感、中度和高度耐药的MRSA菌株及1株MSSA建立小鼠腹膜炎模型，用注射用法罗培南钠及万古霉素通过静脉注射进行感染动物的治疗，评价对感染小鼠的保护作用。结果 151株金葡菌中共分离出65株MRSA，阳性率为43%；体外试验法罗培南和万古霉素对86株MSSA均敏感。65株MRSA对法罗培南敏感者10株，其余均耐药；对万古霉素均敏感。注射用法罗培南钠对4株菌的ED50分别为38.3、38.6、71.9和7.07mg/kg；万古霉素则为2.25、10.18、20.85和1.77mg/kg。结论 注射用法罗培南钠对MSSA有良好的体内和体外抗菌活性；注射用法罗培南钠对MRSA无效，即使体外敏感。 【关键词】 罗培南钠； 金葡菌； 体外抗菌活性； 体内抗菌活性 ABSTRACT Objective To evaluate the antibacterial activities of faropenem sodium for injection against S.aureus in vivo and in vitro. Methods Methicillin resistant S.aureus (MRSA) was screened from clinical isolated S.aureus by cefoxitin disc diffusion method. The minimum inhibitory concentrations (MICs) of faropenem and vancomycin against methicillin sensitive S.aureus (MSSA) and MRSA were determined by agar dilution method. Mice peritonitis models were established by 3 MRSA (sensitive, moderate and severe resistant to faropenem respectively) and MSSA strains and mice were treated introvenously with faropenem sodium and vancomycin for injection. Results Sixtyfive MRSA strains were screened from 151 clinical isolated S.aureus and the positive rate of MRSA was 43%. All MSSA were sensitive to faropenem and vancomycin in vitro. All MRSA were sensitive to vancomycin, but only 10 of them were sensitive to faropenem. In four groups, the median effective dose (ED50) of faropenem were 38.3, 38.6, 71.9 and 7.07 mg/kg respectively; vancomycin were 2.25, 10.18, 20.85 and 1.77 mg/kg respectively. Conclusion The antibacterial activities of faropenem sodium for injection against MSSA in vivo and in vitro are strong. Faropenem is ineffective in vivo to MRSA, even when it was sensitive in vitro. KEY WORDS Faropenem sodium; Staphylococcus aureus; In vivo activity; In