淋巴细胞功能相关抗原-3（CD58）在儿童期急性B淋巴细胞白血病近.docVIP

淋巴细胞功能相关抗原-3（CD58）在儿童期急性B淋巴细胞白血病近 　 作者：赵惠君，蒋黎敏，袁晓军，李莉，汤静燕，沈立松 【摘要】 　　本研究评估CD58在急性B淋巴细胞白血病（B-ALL）近期疗效判断中的价值。应用四色流式细胞分析技术观察135例儿童期B-ALL患者CD58分子的表达特点；建立用CD58/CD10/CD34/CD19抗体组合检测B-ALL MRD的方案；结合CD58的表达情况和MRD监测结果分析CD58在B-ALL中的预后价值。结果表明: 135例B-ALL的平均CD58 MFI为113.08±63.33，15例正常骨髓的CD19+CD10+细胞的平均CD58 MFI为14.68±5.26，两者差异显著（P＜0.01）；51.9%（70/135）B-ALL患者的CD58分子强表达，可以用CD58为指标进行MRD检测；CD58/CD10/CD34/CD19抗体组合的有效频率仅次于TdT/CD10/CD34/CD19，为51.9%；CD58高表达组的MRD阳性率显著低于CD58低表达组（Plt;0.05）。结论： CD58可以作为B-ALL MRD检测的指标，此结果丰富了MRD检测的组合；CD58的高表达可以作为B-ALL预后较好的指标。 【关键词】 急性B淋巴细胞性白血病； CD58； 微小残留病； 淋巴细胞功能相关抗原 　　Prognostic Significance of Lymphocyte Function Associated Anti-gen-3 (CD58) in Childhood B Cell-Acute Lymphocytic Leukemia 　　Abstract This study was aimed to investigate the value of CD58 in evaluation of early therapeutic effect on childhood B-ALL. The expression features of CD58 in 135 cases of childhood B-ALL were analyzed by four-color flow cytometry; MRD detection protocol for B-ALL using CD58/CD10/CD34/CD19 combination was established; the correlation between the expression features of CD58 and MRD detection was analyzed for the early therapeutic response in childhood B-ALL. The results showed that the mean value of CD58 MFI in 135 cases of B-ALL was 113.08±63.33，which was significantly higher than that in 15 cases of normal bone marrow controls (14.68±5.26，P＜0.01). In addition，CD58 was over expressed in 51.9% (70/135) of B-ALL patients，indicating that CD58 could be an effective marker in MRD detection. The CD58/CD10/CD34/CD19 was the second most effective combination next to TdT/CD10/CD34/CD19 in B-ALL MRD detection with flow cytometry. Meanwhile，the positive rate of MRD detection by flow cytometry was significantly lower in CD58 over expression group (Plt;0.05). It is concluded that CD58 may be used as an indicator for detection of MRD in B-ALL patients，which would enriche the combination of MRD detection. The CD58 over expression may be considered as a marker of a favorable prognosis in