灵芝孢子粉对兔移植性肿瘤生存期及外周血影响.docVIP

灵芝孢子粉对兔移植性肿瘤生存期及外周血影响 　 作者：彭玉娜，谭获，王颖超，李智，钟志强，刘汗欣 【摘要】 　　目的探讨灵芝孢子粉对兔移植性肿瘤的生存期及外周血的影响。方法将成功接种荷VX2 乳腺癌兔随机分为灵芝组和对照组，解剖死亡或结束时的兔子，用Kaplan-Meier进行生存曲线分析,Log Rank进行生存曲线显著性检验。比较外周血的变化。结果结束时灵芝组60%生存，中位生存期大于70 d；而对照组只有20%生存，中位生存期为43 d；两组生存期差别有统计学意义(Plt;0.05),兔最终死于远处转移。用药4周后灵芝组的白细胞（17.45×109/L±5.74×109/L）比对照组（11.20×109/L±2.81×109/L）明显升高（P＜0.05）。血红蛋白含量和血小板无明显变化(Pgt;0.05)。结论灵芝孢子粉能够延长荷VX2 乳腺癌兔的生存期，能提高白细胞。 【关键词】 灵芝孢子粉; 移植性肿瘤; 生存期；外周血细胞 　　Abstract：ObjectiveTo study the effect of spores of Ganoderma lucidum on survival time and peripheral blood cell of implanted tumor rabbit .MethodsThe rabbits with transplantable VX2 carcinoma were randomly divided into spores of Ganoderma Lucidum treatment group (10 cases)and control group (10 cases). Survival rate curves were analyzed by Kaplan-Meier method.Difference between survival rate curves was tested by Log Rank test and the changes of Peripheral blood cell were compared between the two groups. ResultsThe survival rate and median survival time in treatment group and the control group were 60%, ＞70day and 20%,43day respectively. There was a significant difference in survival curves. Rabbits were died from distant metastasis. The treatment course lasted 4weeks. The leukocyte count(WBC)of animals in treatment group were（17.45×109/L±5.74×109/L）, which was significantly more than that in control group （11.20×109/L±2.81×109/L）.There has no marked changes in hemoglobin(Hb)and platelet(PLT)(Pgt;0.05).ConclusionSpores of Ganoderma Lucidum can prolong the animals’ survival time and inerease leukocyte count(WBC). 　　Key words：Spores of Ganoderma lucidum; Transplantation animal tumors; Survival time ； Peripheral blood cell 　　灵芝被公认为扶正固本、滋补强身作用的传统中药。灵芝孢子粉是灵芝的精华,其有效成分远远超过了灵芝。大量的研究表明灵芝孢子粉具有抗肿瘤和增强免疫功能作用［1～3］。为进一步研究灵芝孢子粉作用,本实验观察了荷VX2 乳腺癌兔生存期及外周血的影响,为临床用灵芝孢子粉治疗乳腺癌提供依据。 　　1 材料与方法 　　1.1 动物2.0～2.3 kg（10～12周龄）普通级新西兰兔,由广州中医药大学实验动物中心提供及分笼饲养，合格证号为0020557号。 　　1.2 药品灵芝孢子粉由香港HOLISTOL有限公司提供。 　　1.3 瘤株兔VX2瘤株由南方医院惠桥楼罗支农教授惠赠。 　　1.4 方法按文献［4］ 　　要求无菌条件下切取传种兔后腿VX2瘤株实体瘤,生理盐水冲洗