糖皮质激素受体在翼状胬肉中表达及意义.docVIP

糖皮质激素受体在翼状胬肉中表达及意义 　作者：戚朝秀, 王晓黎, 李奇根, 邓娟, 叶辉 【摘要】 【目的】 检测糖皮质激素受体-α、β(GR-α、GR-β)在翼状胬肉组织中的表达，探讨其在翼状胬肉发病机制中的作用。【方法】　取39例翼状胬肉组织（其中初发30例，复发9例），应用荧光定量逆转录PCR定量检测GR-α、β mRNA的表达，应用Western blotting 技术分析相应的蛋白表达，同时通过免疫组化技术分析蛋白的细胞定位。另外，取9例正常人的球结膜作为对照。【结果】　翼状胬肉及正常人的球结膜组织中均表达GR-?琢 mRNA，不表达GR-β mRNA。其中翼状胬肉(39例)中的GR-?琢 mRNA(×103拷贝/μg)为10±4，明显高于正常人球结膜中的2.2±0.3(P＜ 0.01)，经统计检验有显著性差异。但30例初发翼状胬肉与9例复发翼状胬肉组织中的GR-α mRNA表达分别为10±4和10±5, 两者无统计学差异 (P ＞0.05)。Western blotting 分析显示GR-?琢蛋白在翼状胬肉表达显著高于正常人的球结膜组织(P＜ 0.01)。免疫组化显示GR-?琢蛋白定位于上皮细胞核，胬肉组阳性表达率为87.2%，显著高于正常球结膜组(阳性表达率为22.2%，Plt; 0.05)。而GR-β蛋白在两种组织中均不表达。【结论】 翼状胬肉组织中GR-α mRNA及其蛋白表达明显增高，但其在翼状胬肉发生、发展中的作用机制尚待于进一步研究。 【关键词】 翼状胬肉； 糖皮质激素受体-α； 糖皮质激素受体-β Abstract： 【Objective】 The present study investigates whether the glucocorticoid receptor (GR) would be expressed in pterygium. 【Methods】 GR-α and GR-β mRNA expression were detected by a real-time fluorescent quantitative RT-PCR. GR-α and GR-β protein expression were examined by Western blotting analysis and immunohistochemical technique from 39 patients with pterygium (30 primary pterygium and 9 recurrent pterygium). Nine normal conjunctivae were considered as control. 【Results】 The expression of GR-α mRNA (×103 copies/?滋g) were 10±4 in pterygium and 2.2±0.3 in normal conjunctivae. The expression of GR-β mRNA was not detected in pterygium and in normal conjunctivae. There was no difference between the expression of GR-α mRNA in recurrent pterygium （10±5) and the primary pterygium（10±4，P gt;0.05). There was significantly difference between the expression of GR-α mRNA in pterygium and the normal conjunctivae. Western blotting analysis and immunohistochemistry further confirmed these findings for the protein transcripts of these genes. 【Conclusion】 These results demonstrate, for the first time, the expression of GR-α mRNA and protein were higher in pterygium than normal conjunctivae. The role of up-regulation of GR-α mRNA expression in pterygium need more profound investigation. Key words： pterygium; GR-α;