肺腺癌患者血清生物标志物筛选探究.docVIP

肺腺癌患者血清生物标志物筛选探究 　【摘要】 目的 采用SELDI-TOF-MS技术探讨肺腺癌患者血清蛋白标志物，并建立一个稳定、简便、快速的实验方法。方法 肺腺癌患者321例和正常对照人群321例，按照年龄、性别、吸烟史的有无进行1:1配对，采用WCX2芯片技术进行血清蛋白质组学研究，用蛋白芯片阅读器PBSII对芯片进行扫描、分析。结果 肺腺癌患者血清蛋白图谱与正常对照组相比，存在15个差异表达蛋白（Plt;10-5），其中包括11个高表达蛋白，分子量分别是1860Da、4055Da、4195Da、4211Da、4959Da、5329Da、5980Da、6313Da、6880Da、7762Da、9281Da，4个低表达蛋白，分子量分别是2564Da、2932Da、3082Da、3442Da。显著高表达的潜在标志物5个，相对分子量分别为4055Da、4211Da、4959Da、5329Da和7762Da，以峰值比1.5为界值时， 其诊断肺腺癌的灵敏度分别为90.41%、78.08%、50.68%、57.53% 、72.60％，特异度分别为97.06%、93.44%、71.15%、76.36%、94.92％，选用4055Da、4211Da、4959Da、5329Da和7762Da建立决策树模型对样品进行筛选验证，其准确率为85.00%，灵敏度为86.67%，特异度为83.33%。结论 用该技术能筛选出灵敏度和特异性较好的差异表达蛋白，为肺腺癌的早期发现提供了一种新的、无创伤的实验方法，可能具有较好的临床应用前景。 【关键词】 肺腺癌；生物标志物；SELDI－TOF－MS；蛋白质组学 The screening study on lung adenocarcinoma serum biomarkers by SELDI-TOF-MS 【Abstract】 Objective To select relative specific biomarkers in serum from lung adenocarcinoma patients using surface-enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF-MS) protein chip technology.Methods Serum samples from 321 lung adenocarcinoma patients， 321 healthy volunteers with matched gender， age and history of smoking were analyzed using WCX2 potein chip to screening potentially biomarkers. Proteomic spectra were generated by mass spectrometry.Results Five highly expressed potential biomarkers were identified with the relative molecular weights of 4055Da， 4211Da， 4959Da， 5329Da and 7762Da. The sensitivity for diagnosing lung adenocarcinoma was 90.41%， 78.08%， 50.68%， 57.53%， 72.60%; and specificity was 97.06%， 93.44%， 71.15%， 76.36%， 94.92%， when the critical point was made 1.5. The sensitivity for diagnosing lung adenocarcinoma was 86.67%， and specificity was 83.33% by applied to this protein fingerprints pattern validation.Conclusion SELDI-TOF-MS protein chip technology is a quick， easy， convenient， and high-throughput analyzing method capable of selecting several relatively specific， potential biomarkers from the serum of lung adenocarcinoma patients and ma