肾盂输尿管癌细胞增殖及凋亡相关性.docVIP

肾盂输尿管癌细胞增殖及凋亡相关性 　【摘要】 　　目的 探讨Ki67和凋亡(Apoptosis)在肾盂输尿管癌中的表达及其与肿瘤细胞分级、病理分期及预后的关系。方法 采用SABC法和TUNEL法对34例肾盂输尿管癌行Ki67和凋亡检测。结果 Ki67和凋亡在肿瘤细胞分级间存在显著差异，Ki67 index：G2与G1比较Plt;0.05，G3与G2比较Plt;0.05，G3与G1比较Plt;0.01；Apoptosis index：G2与G1比较Plt;0.05，G3与G2比较Plt;0.05，G3与G1比较Plt;0.01。Ki67和凋亡在肿瘤病理分期的T3与T1及T2与T1之间有显著差异，T2与T1比较Plt;0.05，T3与T1比较Plt;0.01，但T3与T2比较Pgt;0.05；Ki67 index以20%为界分为二组，Apoptosis index以3%为界分为二组，组间生存率有显著差异(Plt;0.05)。Ki67在肾盂输尿管癌中的表达与凋亡一致。结论 肾盂输尿管癌细胞增殖与凋亡密切相关；凋亡可作为临床判断肾盂输尿管癌恶性程度、病理分期、细胞分级以及预后的一种客观量化指标。 【关键词】 Ki67　凋亡　肾盂输尿管癌 　　The relationship between proliferation and apoptosis of pelvic ureteral carcinoma 　　ABSTRACT: Objective To study the expression of Ki67 and apoptosis in pelvic ureteral carcinoma and their relationship with the grading, staging and prognosis of tumor. Methods SABC and TUNEL methods were used to examine the expression of Ki67 and apoptosis in 34 cases of pelic ureteral carcinoma. Results Significant difference was present between G2 and G1, G3 and G2 and G3 and G1 of tumer cell in expression of Ki67 and apoptosis, and the P value was less than 0.05, 0.05 and 0.01 respectively in Ki67 index. While in apoptosis index, the value of P was less than 0.05, 0.05 and 0.01 respectively. In all the stages of tumor cell, significant difference was found between T3 and T1, and T2 and T1 in expression of Ki67 and apoptosis tumor cell (Plt;0.01, Plt;0.05), but there was no significant difference between T3 and T2 (Pgt;0.05). Ki67 index was divided into two groups with 20% as the line of demarcation. Apoptosis index was also divided into two groups with 3% as the line of demarcation. And significant difference was present between different groups in survival rate (Plt;0.05). The expression of Ki67 in pelnic ureteral carcinoma was consistant with apoptoins of tumor cell. Conclusion Significant association was present between proliferation and apoptoins in pelvic ureteral carcinoma; and apoptosis may play an important role in clinical evaluation of mali