茯苓超微粉及普通粉挥发性成分探究.docVIP

茯苓超微粉及普通粉挥发性成分探究 　 作者：廖川 ，杨迺嘉，刘建华，黄建城，丁丽娜，袁月玲 【摘要】 目的研究茯苓的挥发性成分。方法利用有机溶剂－水蒸气蒸馏提取茯苓挥发性成分，用GC/ MS进行分离测定，结合计算机检索技术对分离化合物进行结构鉴定，应用色谱峰面积归一化法计算各成分的相对百分含量。结果茯苓超微粉中分离出131个成分，鉴定出67个化学成分，占挥发油总量的51.145%；茯苓普通粉中分离出103个成分，鉴出61个化学成分，占挥发油总量59.223%。茯苓2种粉末挥发油成分中，含有57种相同成分，其中含量最高的分别为α-柏木醇（茯苓超微粉7.936%）、反橙花叔醇（茯苓普通粉8.657%）。结论首次报道利用简便的有机溶剂－水蒸气蒸馏法提取茯苓超微粉挥发性成分，并将其与茯苓普通粉一起进行定性定量的对比研究。 【关键词】 茯苓超微粉； 茯苓普通粉； 有机溶剂－水蒸气蒸馏； 挥发油； 气相色谱-质谱联用 　　Abstract：ObjectiveTo study the chemical constituents of the volatile oil from Poria cocos.MethodsThe chemical compositions of the volatile oil of the plants which were obtained by organic- steam distillation with hexane were analyzed by GC-MS. The constituents were identified by their mass spectra. The relative percentage of the oil constituents was calculated from the GC peak areas. ResultsOne hundred and thirty-one kinds of chemical constituents in micro-milling of Poria cocos were separated,of which sixty-seven compounds representing 51.145% of the oil were characterized. One hundred and three kinds of chemical constituents in ordinary pulverization of Poria cocos were separated,of which sixty-one compounds representing 59.223% of the oil were characterized. There were the same of fifty-seven components in the two oils.The most relative contents was α-Cedrol (micro-milling of Poria Cocos 7.936%)、trans-Nerolidol(ordinary pulverization of Poria Cocos 8.657%). ConclusionThis paper reported, for the first time, the composition of volatile oils of micro-milling of Poria cocos which were obtained by Organic-steam distillation with hexane，which was comparatively studied with ordinary pulverization of Poria cocos about qualitative and quantitative. 　　Key words：Micro-milling of Poria cocos; Ordinary pulverization of Poria cocos; Organic-steam distillation; Volatile oil; GC-MS 　　茯苓为常用中药，始载于《神农本草经》，被列为上品。是多孔菌科真菌茯苓的干燥菌核。味甘淡，性平，有健脾补中，养心安神，利水渗湿的功能。主治水肿尿少，痰饮眩悸，脾虚食少，便溏泄泻，心神不安，惊悸失眠［1］。近年来，茯苓中多糖的成分研究也引起了人们的广泛关注，但其挥发性成分系统性研究未见报道；采用水蒸气蒸馏法对茯苓超微粉、普通粉的挥发性成分提取以及挥发性成分的对比研究，也未见报道。