茴香霉素对小鼠同种异基因皮肤移植影响.doc

茴香霉素对小鼠同种异基因皮肤移植影响 　 作者：陈笛, 邢飞跃, 狄静芳, 陈玲 【摘要】 　　目的: 阐明茴香霉素对淋巴细胞反应性的影响以及在皮肤移植中抑制排斥反应的作用。方法: 用MTT法检测茴香霉素刺激下淋巴细胞在单向或双向混合淋巴细胞反应中的反应性。C57BL/6小鼠作为供体, BALB/c小鼠作为受体。手术前3 d分别给3组BALB/c小鼠皮下注射生理盐水、 环胞霉素A以及茴香霉素, 手术当天继续注射至术后第7天。环胞霉素A及茴香霉素的注射剂量分别为: 20 mg/kg、 12.5 mg/kg。结果: 在剂量10.0 μg/L时, 茴香霉素能抑制单向或双向混合淋巴细胞反应中淋巴细胞的反应性（P均lt;0.01）; 茴香霉素组移植皮肤的存活时间明显高于环胞霉素A组以及对照组。结论: 茴香霉素能明显抑制淋巴细胞的反应性; 并且能明显延长受体对皮肤移植物的耐受时间, 可能为移植免疫排斥的治疗提供新的途径。 【关键词】 茴香霉素 混合淋巴细胞反应 皮肤移植 　　[Abstract] AIM: To investigate the effect of anisomycin on the reactivity of lymphocytes and the rejection in the skin transplantation in mice. METHODS: The reactivity of T cells in unilateral or bilateral mixed lymphocyte reaction was detected by MTT assay. The skins from C57BL/6 mice were transplanted to BALB/c mice and the survival period of the grafts was assessed. The recipients were divided into three groups (physiological saline group, cyclosporin A group and anisomycin group). They were injected with physiological saline (0.1 mL), cyclosporin A (20 mg/kg) and anisomycin (12.5 mg/kg) separately once a day for 3 days before the operation and 7 days after the operation. RESULTS: With the optimal dose of 10.0 μg/L, anisomycin inhibited the reactivity of lymphocyte cells in both unilateral and bilateral mixed lymphocyte reactions. The survival period of the grafts from anisomycin group was longer than that from cyclosporin A group and physiological saline group. CONCLUSION: Anisomycin can inhibit the reaction of lymphocytes and prolong the survival period of the grafts in skin transplantation effectively. It could be used as a new way for transplantation rejection treatment. 　　[Keywords]anisomycin; mixed lymphocyte reaction; skin transplantation 　　茴香霉素是由链霉菌分泌的一种活性成分, 能结合在核糖体60S亚基上阻止多肽链形成。作为临床药物, 茴香霉素已被用于治疗由真菌和阿米巴原虫引起的疾病[1]。最近有研究发现, 茴香霉素与学习、 记忆关系密切[2-4]。然而茴香霉素在免疫系统方面的相关研究很少, 我们在研究中意外发现茴香霉素能明显抑制淋巴细胞的增殖活化（另文发表）, 因此, 将其试用于动物体内研究。本研究以混合淋巴细胞反应为模型测试茴香霉素处理的淋巴细胞在体外对同种异基因抗原刺激的反应性, 以皮肤移植为模型观察其体内对免疫系统的抑制作用。结果显示, 茴香霉素对单向和双向混合淋巴细胞