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a new cultivation method for microbial oil production cell pelletization and lipid accumulation by mucor circinelloides一种新的培养方法微生物石油生产电池粒化和脂质积累的毛霉菌circinelloides.pdfVIP

a new cultivation method for microbial oil production cell pelletization and lipid accumulation by mucor circinelloides一种新的培养方法微生物石油生产电池粒化和脂质积累的毛霉菌circinelloides.pdf

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a new cultivation method for microbial oil production cell pelletization and lipid accumulation by mucor circinelloides一种新的培养方法微生物石油生产电池粒化和脂质积累的毛霉菌circinelloides

Xia et al. Biotechnology for Biofuels 2011, 4:15 /content/4/1/15 RESEARCH Open Access A new cultivation method for microbial oil production: cell pelletization and lipid accumulation by Mucor circinelloides 1,2 2 1 3* Chunjie Xia , Jianguo Zhang , Weidong Zhang and Bo Hu Abstract The recent energy crisis has triggered significant attention on the microbial synthesis of lipids, which comprise the raw material for biodiesel production. Microbial oil accumulation with filamentous fungi has great potential because filamentous fungi can form pellets during cell growth, and these pellets are much easier to harvest from cell broth. This paper focuses on the cell pelletization process of the oleaginous Mucor circinelloides. We have studied the effect of various cultural conditions on pelletized cell growth and lipid accumulation. This study is the first to report that pH adjustment during cell growth plays a key role in pellet formation of M. circinelloides and describes a handy method by which to induce cell pelletization in submerged fungal cultivation. Our study reveals that cell growth and lipid production are not significantly affected by pelletization and that lipid accumulation is triggered at stressed conditions, such as a high carbon-to-nitrogen ratio and high temperature. Background By utilizing glycerol, acetic acid, soluble starch, wheat Biomass-based biofuel production has emerged as a straw, wheat bran and so forth, some oleaginous filamen- major approach to enabling energy independence, redu- tous fungi can be used to produce lipids [12-15]. The cing greenhouse gas emissions, revitalizing rural com- capabil

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